Lin, Hsin-HungHsin-HungLinWang, Chih-YenChih-YenWangHsieh, Feng-JenFeng-JenHsiehLiao, Fang-ZhenFang-ZhenLiaoSu, Yu-KaiYu-KaiSuPham, Minh DinhMinh DinhPhamCHIH-YUAN LEEChang, Huan-ChengHuan-ChengChangHSAO-HSUN HSU2023-11-282023-11-282023-0717435889https://scholars.lib.ntu.edu.tw/handle/123456789/637399Background: The use of nanodiamonds (NDs) and fluorescent nanodiamonds (FNDs) as nonallergenic biocompatible additives in incomplete Freund's adjuvant (IFA) to elicit immune responses in vivo was investigated. Methods: C57BL/6 mice were immunized with chicken egg ovalbumin (OVA) in IFA and also OVA-conjugated NDs (or OVA-conjugated FNDs) in IFA to produce antibodies. OVA-expressing E.G7 lymphoma cells and OVA-negative EL4 cells were inoculated in mice to induce tumor formation. Results: The new formulation significantly enhanced immune responses and thus disease resistance. It exhibited specific therapeutic activities, effectively inhibiting the growth of E.G7 tumor cells in mice over 35 days. Conclusion: The high biocompatibility and multiple functionalities of NDs/FNDs render them applicable as active and trackable vaccine adjuvants and antitumor agents.enadjuvantsantitumor agentsfluorescent nanodiamondsmiceovalbuminvaccine[SDGs]SDG3journal article10.2217/nnm-2023-0179376100042-s2.0-85170110370