Huang J.-G.Hung C.-C.K.HSIN-CHIH LAILee C.-K.SHI-MING LINFeng P.CHII-WANN LIN2020-07-032020-07-0320040589-1019https://scholars.lib.ntu.edu.tw/handle/123456789/508271Tuberculosis is one of notifiable infectious diseases which may cause serious epidemic problems. Traditional diagnostic techniques include acid- fast stain, bacteria culture, metabolic monitoring have some disadvantages such as low sensitivity, time consumption and less specificity. In this study we proposed a new diagnostic technique based on the protein chips concept which detected by SPR phenomena. It was found that the resonance angle (?51.86°) shifted slightly toward right with antibody concentration of 10X, 30X, 100X, and 300X dilution under optimal concentration of immobilized TB antigen W38 (MW 41.5 kDa, 50 μg/ml). While for the W06 (MW 14 kDa, 50 μg/ml), the resonance angle was around 50.13° and shifted with the same trend as W38 did.[SDGs]SDG3Antibodies; Bacteria; Concentration (process); Diseases; Molecular biology; Monolayers; Photoresists; Proteins; Biochips; Glass substartes; Protein chips; Tuberculosis; Surface plasmon resonanceconference paper2-s2.0-11144286044