2021-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/682857Exercise is beneficial for human health and also prevents development of cancer, metabolic diseases and cardiovascular diseases. Exercise not only prevents cancer initiation, but also delays cancer progression and improves cancer therapeutic effects. However, the molecular mechanism of how exercise delays cancer progression is still not clear. Many exercise-induced factors, including cytokines, peptides, and hormones, have been proposed as the key for delaying cancer progression. None of them had the concrete results yet. Here, we propose that irisin, the exercise-induced myokine, plays a key role in cancer prevention and is one of reasons why exercise delays cancer development. Moreover, because lower gene expression of FNDC5, the precursor of irisin, in glioblastoma patients, in this study, the irisin effects on glioblastoma are investigated. Glioblastoma is the most common and aggressive brain tumor type, and the survival rate of glioblastoma is less than one year. Hence, the new therapeutic approach is in urgent need. First, we observe that the purified recombinant human irisin reduced glioblastoma cell growth due to cell cycle arrest. Next, irisin also inhibits glioblastoma cell invasion. Moreover, to decipher the detailed molecular mechanism of irisin effects on glioblastoma cell proliferation and cell invasion, several genes selected from transcriptome analysis will be further investigated. Last but not the least, the above results will be further verified in in vivo tumor models. The results of the project help us to develop the new strategy for cancer therapy and decipher the molecular mechanism of how exercise prevents cancer development.Exercise, myokine, irisin, glioblastoma, cell invasion