2008-11-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/695574摘要：由於具高殺蟲效果及對於哺乳類動物低毒性的特性，類尼古丁殺蟲劑被廣泛地應用於農業蟲害防治。然而此類藥劑卻可能造成蜜蜂導航神經機制的失常，引發蜂群衰竭失調症等問題。為釐清蜂群衰竭失調症的肇因，本研究以益達胺為對象，使用電生理研究方法及學習行為測試評估，研究其急性及慢性藥害對蜜蜂導航神經機制的影響，進而探討此類藥劑的施用與蜂群衰竭失調症肇因的關聯。由於蜜蜂導航機制中，磁感受運用的神經機制仍全然未知；因此我們亦將進行相關的電生理及其神經迴路結構研究，增進對蜜蜂磁感受機制的認知，以延伸農藥危害評估涵蓋的層面。此外本研究亦將探討農藥毒害是否可能與病原感染的影響互相加成，形成複合因子，加速蜂群衰竭失調症的爆發。最後我們將比對研究中所發現的危害現象是否真實發生於罹患蜂群衰竭失調症的蜂群內，以瞭解農藥毒害與蜂群衰竭失調症之關聯。<br> Abstract: Since the toxicity of neonicotinoids is highly specific to insects, these insecticides are frequently used in agricultural environment. However, these insecticides have been suspected as the causes to induce neural disorder of honeybee’s navigation system, and thus cause the symptom of colony collapse disorder (CCD) of the honeybee. In this study, we will examine the hypothesis by evaluating the acute and chronic toxicities of a widely used neonicotinoid, imidacloprid. Both the acute and chonic toxicities will be studied electrophysiologically in the honeybee nervous system. The chronic toxicity will be further investigated by evaluating the learning abilities of poisoned honeybees. While the mechanism of magnetoreception in the honeybee is still unknown, and the manetism has been considered as one of the main navigational cues for the bee workers, this study will also explore the neural processing and architecture of the magnetoreception system of honeybee. The study of magnetoreception will help us to clarify whether the navigation disorder in CCD is caused by neonicotinoid poisoning. Because the insecticidal toxicity may syngerize the the pathogenic infections and therefore cause a complex colony disorder as observed in CCD, the possiblity will be also examined. All the experiments in this project will be verified with the CCD bees to determine the primiary fators causing the bee lost as found in the CCD.蜂群衰竭失調症導航磁場感應類尼古丁殺蟲劑蜜蜂colony collapse disorderCCDnavigationmagnetoreceptionneonicotinoidshoneybeeApis mellifera