SANG-HENG KOKYeh C.-C.Chen M.-L.YEN-PING KUO2021-07-052021-07-0520091368-8375https://www.scopus.com/inward/record.uri?eid=2-s2.0-70449518175&doi=10.1016%2fj.oraloncology.2009.07.018&partnerID=40&md5=07a79c110c888997fc2cb6f13453d4d5https://scholars.lib.ntu.edu.tw/handle/123456789/569183Esculetin has been shown to selectively induce tumor apoptosis in several types of cancers and is regarded as a promising chemotherapeutic agent. In this study, we showed that esculetin significantly suppressed the growth of oral cancer SAS cells in a dose-dependent manner. DNA content flow cytometry and TUNEL assay revealed that esculetin induced cell cycle arrest and apoptosis. Western blotting showed esculetin increased DR5 protein expression and activated caspase-8, which differed from previous studies conducted in other cell types. Furthermore, treatment with esculetin significantly increased TRAIL-induced apoptosis in SAS cells and the TRAIL-sensitizing effect was blocked by DR5/Fc chimera protein. Our results indicate that esculetin enhances TRAIL-induced apoptosis primarily through upregulation of DR5. Combination of esculetin and TRAIL may be a novel treatment strategy for oral cancers. ? 2009 Elsevier Ltd. All rights reserved.[SDGs]SDG3caspase 8; death receptor 5; DNA; esculetin; tumor necrosis factor related apoptosis inducing ligand; apoptosis; article; cancer cell; cancer growth; cell cycle arrest; cell growth; cell type; controlled study; dose response; enzyme activation; flow cytometry; human; human cell; mouth cancer; nick end labeling; priority journal; protein expression; Western blotting; Antioxidants; Apoptosis; Blotting, Western; Caspase 8; Cell Cycle; Flow Cytometry; Humans; In Situ Nick-End Labeling; Mouth Neoplasms; Neoplasm Proteins; Receptors, TNF-Related Apoptosis-Inducing Ligand; Umbelliferonesjournal article10.1016/j.oraloncology.2009.07.018197205572-s2.0-70449518175