鄭玉娟2006-07-252018-06-292006-07-252018-06-292005-07-31http://ntur.lib.ntu.edu.tw//handle/246246/4707DNA 修補基因多形性與癌症的發生，是 近年來分子生物研究重視的課題之一，本研 究藉由分子流行病學方法，首度探討DNA 的修補基因NQO1 、XPD156 基因多形性與 環境因子共同作用時，發生鼻咽癌之關係。 中國人之鼻咽癌發生率居全世界之 冠，比白人高出20 到30 倍，亦高出日本人 或韓國人甚多。相較於其他癌症，鼻咽癌是 屬較早發之癌症且好發於男性，一旦發病對 社會之衝擊是不可忽視的。本計劃將著重於 國人特有之鼻咽癌危險因子之分析，並以病 例對照研究探討鼻咽癌環境致癌物與基因之 交互作用。其研究對象是來自民國80 年7 月 至83 年12 月，由台大醫學院、台大公共衛 生學院與美國國立癌症研究院共同合作之鼻 咽癌病例對照研究，在台大及馬偕醫院共收 集355 名經病理確診之新病例,年齡在75 歲 以下,居住在臺北市/縣六個月以上，283 名經 由年齡（同-5 歲年齡組）、性別、居住地區配 對之社區對照，將所收集之檢體利用白血球 去氧核糖核酸以聚合酵素連鎖反應為基礎的 限制片段長度多形性(PCR-RFLP)進行DNA 修復基因NQO1 、XPD156 之多形性測定。 綜合以結構式問卷進行標準化訪視所 蒐集可能致癌之危險因子，以非條件式對數 複回歸分析進行模型建構，做多變項分析， 在調整性別、年齡、抽煙、種族及教育狀況， 結果發現NQO1 、XPD156 的基因多形性與鼻 咽癌發生的危險並無顯著相關。 我們進一步分別以抗EBV 抗體 （anti-EBV antibodies ）、木頭粉塵及甲醛 （formaldehyde ）的職業曝露及亞硝月安 （nitrosamine ）的飲食攝取和抽煙曝露做分 層分析。結果顯示鼻咽癌危險性在女性、未 抽煙者和NQO1 多形性有顯著相關，在45 歲以下的人和NQO1 多形性有邊緣性相關； 而在XPD156 的多形性間則不具相關。Genetic polymorphisms of DNA repair genes have been reported to determine susceptibility to several cancers. This study focused on effects of genetic polymorphisms of DNA repair genes NQO1 and XPD156 interacted with environmental factor on the development of nasopharyngeal carcinoma(NPC). The incidence of nasopharyngeal carcinoma in Chinese is the highest in the world. It is 20-30 times compared to Caucasian, and also much more than Japanese and Korean. Compared to other cancers, the peak of nasopharyngeal carcinoma more frequently occurs in younger age and dominant in male. The impact of the disease especially can not be ignored. The specific aim of this study is to explore the risk factors of nasopharyngeal carcinoma (NPC). A case-control study for NPC which was collaborated with College of Medicine, College of Public Health National Taiwan University and the National Institute of Health in America was conducted in Taiwan from July 1991 to December 1994 to investigate the interaction of environmental carcinogen and genotype polymorphism to the risk of NPC. We enrolled 355 histologically confirmed incident NPC cases who were younger than 75 years old, and had resided in Taipei city/county for at least 6 months, from National Taiwan University Hospital and Mackay Memorial Hospital. Totally 283 healthy community controls were selected by individual matching for sex, age and residence. The study will use the real-time polymerase chain reaction (RTPCR) to analyze the geno types of DNA repair genes NQO1 and XPD156. Information of Risk factors were obtained through personal interview with structured questionnaire, in which included demographic factors, the history of smoking, drinking, dietary habit, family history of cancer etc. The information were combined with genotyping results to evaluate the effect of NPC development. Unconditional logistic regression was performed to clarify the relationship between various risk factors, genetic susceptibility and NPC occurrence, and also to elucidate their interaction. After adjusting age, sex, ethnicity, educational level, and duration of smoking, NPC is not associated with genetic polymorphisms of NQO1 and XPD156. We further stratified by anti-EBV antibodies, occupational exposure to wood dust and formaldehyde, and dietary nitrosamine intake. When stratified 0-10 years exposure to either formaldehyde or wood dust, NPC risk is significantly correlated with NQO1 polymorphism, while remained no significant association between NPC risk and XPD156.application/pdf151019 bytesapplication/pdfzh-TW國立臺灣大學公共衛生學院流行病學研究所鼻咽癌病例對照研究危險 因子DNA 修補基因基因多 形性易感受性NQO1XPD156nasopharyngeal carcinomacase-control studyrisk factorDNA repair genesgenetic polymorphismsusceptibility[SDGs]SDG3reporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/4707/1/932320B002085.pdf