Chao, C.-H.C.-H.ChaoChou, K.-J.K.-J.ChouHuang, C.-Y.C.-Y.HuangWen, Z.-H.Z.-H.WenHsu, C.-H.C.-H.HsuWu, Y.-C.Y.-C.WuCHANG-FENG DAISheu, J.-H.J.-H.Sheu2018-09-102018-09-102012http://www.scopus.com/inward/record.url?eid=2-s2.0-84863287330&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/370871One new sterol, crassarosterol A (1), and four new steroidal glycosides, crassarosterosides A-D (2-5) were isolated from the Formosan soft coral Sinularia crassa. The absolute configuration of 1 was determined using the Mosher's method. The absolute configurations for the sugar moieties of 2-5 were determined by HPLC analysis on the o-tolylthiocarbamates derived from the liberated sugar after acid hydrolysis. Compounds 2 and 4 could significantly inhibit the expression of pro-inflammatory iNOS protein at 10 ?M. In contrast, 1-3 were found to stimulate the expression of COX-2 protein at this concentration. Steroids 1 and 4 also showed cytotoxicity toward the selected human liver cancer cells. ? 2012 by the authors; licensee MDPI.Anti-inflammatory activity; Crassarosterol A; Crassarosterosides A-D; O-tolylthiocarbamate; Sinularia crassa[SDGs]SDG3crassarosterol A; crassarosteroside A; crassarosteroside B; crassarosteroside C; crassarosteroside D; cyclooxygenase 2; inducible nitric oxide synthase; natural product; steroid; unclassified drug; antiinflammatory activity; antineoplastic activity; article; cancer cell; concentration response; controlled study; coral; drug cytotoxicity; drug determination; drug hydrolysis; drug isolation; drug structure; high performance liquid chromatography; human; human cell; liver cancer; protein expression; Sinularia crassa; structure analysis; Alcyonacea; Sinulariajournal article10.3390/md100204392-s2.0-84863287330WOS:000300716000012