2023-08-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/653930麻竹為臺灣分布最廣之竹類，生長快速，為本土竹類單一生長季生長量最大者。麻竹富含醣類及纖維，且隨生長時間的增加而快速累積，為生質能源及工業利用的高潛力材料。然而麻竹為禾本科植物，而禾本科植物細胞壁中含有多量以酯鍵與半纖維素相連之羥基肉桂酸。這些化學結構形成木質素碳水化合物複合體影響後續生質能利用甚劇。本年計畫目的為選殖出麻竹羥基肉桂酸轉移酶相關基因，並確認這些基因的表現量；同時，也將試著體外表現這些蛋白質，作為進行後續年度計畫相關試驗之用。預期完成之計畫成果可對麻竹木質素碳水化合物複合體生合成提供重要資訊。未來可據以改良麻竹的培育與生長，使其成為適合特殊用途的優良生質材料，進而擴展麻竹的經濟效益及利用領域。 Ma bamboo is the most widely distributed bamboo species in Taiwan. It grows extremely fast, and the newly-grown biomass within one growing season is the most significant among other local bamboo species. Ma bamboo is rich in polysaccharides and fibers, and they accumulate quickly during growth. These make ma bamboo a potential raw material for bioenergy and industry purposes. However, ma bamboo belongs to Poaceae, and the characteristics of Poaceae hemicelluloses are rich in ester-linked hydroxycinnamic acids. These chemical structures would form lignin carbohydrate complexes, and affect later bioenergy applications. The objectives of the current proposal are to clone these acyltransferase-related genes from ma bamboo and to profile the expression levels of these genes. The candidate genes will be expressed in vitro to be used in the experiments of the future year. The results from this proposal could provide vital information to the biosynthesis of lignin carbohydrate complexes in ma bamboo. We will be able to extend the basic knowledge obtained from this research to improve the cultivation of ma bamboo and to excel ma bamboo as a promising biomass species for many industrial applications.麻竹;半纖維素;羥基肉桂酸醯基轉移酶;Dendrocalamus latiflorus Munro; hemicelluloses; hydroxycinnamic acid acyltransferase