蔡詩偉Tsai, Shih-Wei臺灣大學:環境衛生研究所李婉甄Lee, Wan-ChenWan-ChenLee2010-05-072018-06-302010-05-072018-06-302008U0001-2807200815293000http://ntur.lib.ntu.edu.tw//handle/246246/181417本研究利用固相微萃取(Solid-Phase Microextraction, SPME)技術免溶劑脫附及操作簡單操作等優點，發展以動態暴露系統採樣之方法，同步偵測多種室內常見的MVOCs。研究選取了台灣室內常見的七種MVOCs，包括2-methyl-1-propanol, 1-butanol, 3-methyl-1-butanol, 2-hexanone, 2-heptanone, 1-octen-3-ol 及 2-pentylfuran，分別以PDMS/DVB、DVB/Carboxen/PDMS、Carboxen/PDMS三款SPME纖維置入暴露腔，在固定的氣體流速下，改變採樣時間及採樣濃度等條件，進行外推式採樣；採樣之後將纖維置於氣相層析儀之注射口進行熱脫附，接著以質譜儀進行分析。研究結果發現，採樣時間對纖維吸附的MVOCs有顯著影響。在固定MVOCs濃度下，當採樣時間超過某一臨界值，MVOCs之間有競爭效應的發生。但在此臨界時間範圍之內，採樣時間及採集質量間有良好的線性關係，顯示隨著採樣時間增加，纖維上採集的質量也跟著增加。另外，在經過不同範圍之MVOCs採樣濃度測試，發現以Carboxen/PDMS纖維在0.8μg/m3 to 8 μg/m3濃度下進行40分鐘的採樣，沒有競爭效應的發生；而所得之採樣率分別為2-methyl-1-propanol 0.0884±0.0038 cm3/s、1-butanol 0.1231±0.0024 cm3/s、 3-methyl-1-butanol 0.0268±0.0008 cm3/s、2-hexanone 0.0355±0.0005 cm3/s、2-heptanone 0.1379±0.0062 cm3/s、1-octen-3-ol 0.0253±0.0044 cm3/s及2-pentylfuran 0.0748±0.002 cm3/s。研究所發展之動態採樣系統結合Carboxen/PDMS纖維之使用，除了有免溶劑脫附及採樣時間短等優點，亦具有未來應用於室內環境MVOCs偵測之潛力。More emphasis has been placed on mold in recent years because exposure to indoor mold can cause adverse health effects in human. Microbial Volatile Organic Compounds (MVOCs) are chemicals generated by molds during their life cycle and can be used as markers for mold growth indoors because they can diffuse through building materials which can not be penetrated by mold spores. And positive associations have also been found between the exposures of MVOCs and asthma or allergy. Therefore, the objective of this research was to develop a dynamic sampling method which is rapid and sensitive enough for the determination of MVOCs with solid-phase microextraction (SPME) technique.hree SPME fibers were evaluated in this study for their suitability in sampling seven commonly seen mold indoors in Taiwan, including 2-methyl-1-propanol, 1-butanol, 3-methyl-1-butanol, 2-hexanone, 2-heptanone, 1-octen-3-ol, and 2-pentylfuran. The dynamic sampling system which produced a constant flow of known concentration of MVOC vapor was used for rapid sampling. After sampling, the SPME fiber was inserted to the injection port of gas chromatograph with mass spectrometer (GC/MS) for thermal desorption and further analysis.esults showed that the Carboxen/PDMS fiber was found to be the most suitable among the three fibers for MVOCs sampling. Sampling with Carboxen/PDMS also presented a good linearity between mass collected on fiber and the magnitude of exposure (in concentration-time units). In addition, when sampling for 40 min from 0.8 μg/m3 to 8μg/m3, no competitive adsorption occurred. And the experimental sampling rates were 0.0884±0.0038 cm3/s for 2-methyl-1-propanol, 0.1231±0.0024 cm3/s for 1-butanol, 0.0268±0.0008 cm3/s for 3-methyl-1-butanol, 0.0355±0.0005 cm3/s for 2-hexanone, 0.1379±0.0062 cm3/s for 2-heptanone, 0.0253±0.0044 cm3/s for 1-octen-3-ol, and 0.0748±0.002 cm3/s for 2-pentylfuran.his study demonstrated the potential of using Carboxen/PDMS fiber for analysis of MVOCs under nonequilibrium conditions, which possesses the advantages of the SPME technique. And the sampling rates determined in this study can possibly be applied in the future for field study.誌謝 i文摘要 iiBSTRACT iiiABLE OF CONTENTS ivIST OF TABLES viiIST OF FIGURES viiiHAPTER 1 INTRODUCTION 1.1 INDOOR AIR QUALITY 1.2 INDOOR MOLDS 1.2.1 MOLDS AND RELATED HEALTH PROBLEMS 2.2.2 MVOCS PRODUCED BY MOLDS 3.2.3 COMMON INDOOR MOLDS AND RELATED MVOCS IN TAIWAN 4.3 INDOOR MOLD DETECTION 4.3.1 BIOLOGICAL DETECTION 5.3.2 CHEMICAL DETECTION 6HAPTER 2 SOLID-PHASE MICROEXTRCTION, SPME 8.1 INTRODUCTION TO SPME TECHNIQUE 8.2 COATINGS OF SPME FIBERS 9.3 AIR SAMPLING UNDER NON-EQUILIBRIUM CONDITIONS 10.3.1 EQUILIBRIUM AND NON-EQUILIBRIUM CONDITIONS 10.3.2 INTERFACE CALIBRATION THEORY 11.3.3 COMPETITIVE ADSORPTION AND DISPLACEMENT EFFECT 17HAPTER 3 RESEARCH OBJECTIVES AND STRUCTURE 18.1 RESEARCH OBJECTIVES 18.2 RESEARCH STRUCTURE 19HAPTER 4 MATERIALS AND METHODS 20.1 CHEMICALS 20.2 STANDARDS 20.3 DYNAMIC VAPOR GENERATION SYSTEM 21.3.1 SET UP OF THE SYSTEM 21.3.2 SYSTEM STABILITY TEST 22.3.3 VERIFICATION OF SAMPLING AIR VELOCITY 22.3.4 CABRATION OF THE SYRINGE PUMP INJECTION RATE 24.4 DYNAMIC SAMPLING OF MVOCS 24.5 INSTRUMENTAL ANALYSIS 25.6 SPME FIBERS 26HAPTER 5 RESULTS AND DISCUSSIONS 27.1 EFFECT OF SAMPLING VELOCITY ON MASS COLLECTED ON FIBER 27.2 COMPARISON OF FIBER PERFORMANCES 28.2.1 EFFECTS OF THE COMPETITIVE ADSORPTION ON THE ADSORBED MASS 28.2.2 SAMPLING RATES 29.2.3 CORRELATION BETWEEN PREDICTED AND MEASURED MASS 30.3 SAMPLING WITH CARBOXEN/PDMS FIBER UNDER LOW SAMPLING CONCENTRATIONS 31.3.1 COMPETITIVE ADSORPTION 31.3.2 SELECTION OF OPTIMAL RANGE OF SAMPLING TIME 31.4.1 SAMPLING RATES 32.4.2 CORRELATION BETWEEN PREDICTED AND MEASURED MASS 33HAPTER 6 CONCLUSIONS 34EFERENCES 35application/pdf839956 bytesapplication/pdfen-US固相微萃取MVOCs動態暴露系統氣相層析質譜儀solid-phase microextractionnonequilibrium sampling conditionGC/MS[SDGs]SDG3thesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/181417/1/ntu-97-R95844006-1.pdf