Ho-Hsiung ChangYuh TzeanHsin-Hung Yeh2025-03-062025-03-062024https://www.scopus.com/record/display.uri?eid=2-s2.0-85199936126&origin=recordpagehttps://scholars.lib.ntu.edu.tw/handle/123456789/725491Phloem-specific promoter efficiently triggers graft-transmissible RNA interference (gtRNAi). We leveraged a phloem-specific promoter derived from the Rice tungro bacilliform virus, optimizing the RNAi mechanism’s efficiency and specificity. Here, we detail the construction of phloem-specific promoter-based gtRNAi system and its application through grafting experiments, demonstrating its effectiveness in inducing tomato yellow leaf curl Thailand virus (TYLCHTV) resistance in non-transgenic scions. This strategy presents a practical application for protecting crops against viruses without genetically modifying the entire plant.Graft-transmissible RNA interference (gtRNAi)Phloem-specific promoterRice tungro bacilliform virus (RTBV)RNA interference (RNAi)Tomato yellow leaf curl Thailand virus (TYLCHTV)[SDGs]SDG2book part10.1007/978-1-0716-4063-0_16