楊泮池2006-07-262018-07-112006-07-262018-07-111999http://ntur.lib.ntu.edu.tw//handle/246246/23417呼吸道之黏液醣蛋白是分子量相當大 （>200 KD ）的醣化蛋白，覆蓋於呼吸道上 皮細胞之表面，有保護和潤滑的作用。而 急性呼吸窘迫症候群(ARDS )是肺部相當 嚴重且廣泛的傷害，由於各種發炎細胞及 介質的作用，造成肺泡及肺泡微血管破 壞。病人呈現呼吸衰竭，且有極高的死亡 率。但一直沒有好的生物標記來預測急性 呼吸窘迫症候群的發生及預後。我們先前 的研究發現，急性呼吸窘迫症候群病人血 清中，含有比正常人（吸菸、非吸菸群）、 慢性支氣管炎患者、心因性肺水腫病人較 高的黏液醣蛋白相關抗原，且血清黏液醣 蛋白值與ARDS 病人之肺傷害指標有相當 好之相關性，而ARDS 病人血清中大分子 之黏液醣蛋白的比例減少，被分解之小分 子黏液醣蛋白增加。所以我們設計這個計 畫，利用特異單株抗體免疫化學染色評估 肺組織中黏液醣蛋白的含量及分佈。想進 一步證實急性呼吸窘迫症候群之肺組織中 黏液醣蛋白特異基因的表現型態，以了解 在發炎反應對黏液醣蛋白基因表現的影響。Mucins are high molecular weight glycoproteins (MW>200kD) present in the human airway with the function of protection and lubrication. Acute respiratory distress syndrome (ARDS) represents a severe, diffuse lung injury caused by a wide variety of insults. The overwhelming inflammatory cells and mediators result in disruption of the alveolocapillary membrane, and leakage of a protein-rich fluid into the alveolar airspaces. Our previous study showed serum levels of mucin-associated antigen elevated in patients with ARDS. Serial measurements of serum mucin levels were inversely correlated with static respiratory system compliance and log (PaO2/FiO2), and positively correlated with lung injury score. The serum mucins of patients with ARDS were polydispersed and smaller than the antigens in normal sera. We hypothesize that elevated serum mucin is due to increased production, increased permeability or increased degradation to smaller molecules before or after they gain access into blood stream. In this project, we used immunohistochemical stains to evaluate mucin expression in lung tissue of patients with ARDS in comparison with the values of control groups. We want to compare the pattern of mucin genes expression in ARDS and normal lung tissue; as a basis to study the mucin genes expression in inflammation.application/pdf33520 bytesapplication/pdfzh-TW國立臺灣大學醫學院內科黏液醣蛋白急性呼吸窘迫症候群MucinsAcute Respiratory Distress Syndromereporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/23417/1/882314B002206.pdf