2014-09-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/651655摘要：細胞集體移動在許多的生理過程中扮演了很重要的角色，如胚胎發育、組織受傷後細胞重新生長、以及癌細胞移動。組織受到外來的因素而導致細胞脫落或受損形成傷口，為了修復傷口，組織會讓細胞遷移和增長，這時就會有細胞集體移動的現象出現。為了研究這個現象，許多傷口癒合的實驗陸續被研究人員創造出。這眾多的傷口癒合實驗都是基於利用機械性、化學性、光學、或電學的方法在單層的細胞膜中創造一個傷口，也就是沒有細胞的區域。這些實驗主要是用來研究細胞在受到不同的物理或化學刺激後的遷移過程。這些製造出來的傷口會隨著不同的方法而有所差異，就算是同一種的方法也有可能造成差異，例如刮掉細胞的機械性方法。在這個的計畫中，我們將利用光學的方法—UV光，選擇性地將細胞殺死並在單層細胞膜中製造出一個傷口。主要使用的UV光是UVB和UVC，我們還會將UV光方法與傳統的刮掉細胞方法做比較。另外，鑑於低功率雷射光治療方法的出現，它可促進傷口快速以及有效的癒合，我們計畫利用UV光來照射傷口，看是否可以加速傷口癒合的速度。我們將設計及製造一微流道晶片來使不同強度及波段的UV光照射於傷口上。微流道晶片提供細胞對微環境反應的體外研究平台，它能夠精準地控制細胞所處環境的溫度、化學分子濃度、pH值、剪應力、及鹽類離子濃度等相關變因。這實驗的主要目的是希望能找出一個最佳的條件，能有效地利用UV光來加速傷口的癒合。<br> Abstract: Collective cell migration plays important roles in many physiological processes such as embryonic development, tissue repair, and angiogenesis. A “wound” occurs when epithelial cells are lost and/or damaged due to some external factors, and collective cell migration occurred in the following wound-healing process. To study this collective cellular behavior, many wound-healing assays are developed. In these assays, a “wound”, or a “cell-free region”, is created in a monolayer of cells mechanically, chemically, optically, or electrically. These assays are designed to study the effects of certain stimuli, physical or chemical, on cellular migration. However, different methods may result in wounds with different sizes or depths, and even one method will give batch-to-batch variation. For example, in a mechanical scratch assay, it is not easy to precisely control the size of the wound from experiment to experiment. In this proposal, we will use UV lights (UVB and UVC) to selectively kill cells and create a wound out of a monolayer. A comparison will be made between UV light wound-healing assay and traditional scratch assay. Also, in view of the development in low level light irradiation (LLLI), we will study effects of UV lights on wound healing. A microfluidic chip will be designed and fabricated to allow UV lights with different wavelengths and intensities shining on wounds. Microfluidic chips provide an in vitro platform for studying cells’ responses to their micro-environments, where the temperature, concentrations of chemical molecules and salts, pH, shear stress, and other related parameters could be well controlled. We hope to find an optimal UV dose that can help in accelerating wound healing.UV光傷口癒合實驗微流道晶片光治療UV lightsWound-healing assayMicrofluidic chipsLight therapy