2014-08-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/658859摘要：人體使用之醫療器材如植體、心導管、導尿管等會因細菌貼附，導致慢性感染，造成組織發炎，或是需要更換，浪費許多醫療資源。聚甲基丙烯酸甲酯常用於活動假牙、矯正維持器，而導尿管置入常會發生泌尿道感染，至今都還沒有有效抑制細菌貼附的方法，雖然已有許多研究關於生醫材料表面改質接枝，以抗細菌或抗蛋白質貼附，如接枝親水性單體 HEMA，用化學聚合、加熱法或電漿接枝等，改質試片表面，進一步研究抗細菌貼附或生物相容性情況等。但其 HEMA 造成抗貼附機制還不明確，但大多認為可能是因為親水性與表面特性改變，而利於改善細菌或細胞貼附的情形。本實驗使用牙科臨床上製作維持器材料，為自聚合式聚甲基丙烯酸甲酯以及常用在ureteral stents 的 polyurethane。第一年藉由不同接枝材料與方法改質疏水性 PMMA 和polyurethane表面，如加熱接枝 HEMA、電漿接枝 PEGMA或 PEGDA。比較彼此之間抗細菌貼附情況，再加入超親水 silicon及超疏水 Teflon並搭配各組平滑和粗糙表面，探討表面親疏水性和物理性質粗糙度的改變，是否為主要引起減少細菌貼附原因。第二年以動物實驗比較PMMA, PMMA-HEMA, PMMA-PEGMA, PMMA-PEGDA用於口腔和導尿管是否具有抗菌功能。第三年的主要目標為探討抗細菌貼附的機制。藉由此三年之計畫，期望能瞭解抵抗細菌貼附的有效方法，增進民眾之健康。<br> Abstract: Persistent biofilm formation on medical devices such as implants, catheters, and ureteralstents can cause chronic infection and often result in replacement finally. Therefore,development of new strategy or materials to combat biofilm formation has been an importanttopic of research. Polymethyl methacrylate (PMMA) is generally used in dentistry for denturebase materials, maxillofacial prostheses, temporary restoration, and orthodontic appliances.Utilization of urinary catheters is associated with some adverse effects, including increasedpropensity to develop bacteriuria and symptomatic infection. There is not an effective methodto modify PMMA and urinary catheters surfaces against bacterial adhesion.This project will use PMMA and polyurethane (PU) as the substrates. The first year willinvestigate the effects of hydrophylicity and roughness on bacterial adhesion. PMMA and PUare grafted with pHEMA, PEGMA, and PEGDA to evaluate the ability of antibacterialadhesion. ATR-FTIR, XPS, SEM, contact angle, and surface roughness measurement will beperformed to analyze the surface properties. The second year will use pigs and rabbits asanimal models to test the surface modification of PMMA and PU against bacterial adhesion.The third year is scheduled to investigate the mechanism of surface grafting against bacterialadhesion. We hope to develop an effective method to resist bacterial adhesion through theperformance of this three-year project.聚甲基丙烯酸甲酯2-甲基丙烯酸羥乙酯聚乙二醇異丁烯酸聚乙二醇雙丙 烯酸酯細菌PMMAHEMAPEGMAPEGDAbacteria