2020-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/698377For overcoming the food safety issue, non-targeted metabolomics is an effective approach in the detection of contaminants in food. Aims of this study are focus on developing a comprehensive analysis to investigating both the good nutrients and possible bad hazard compounds in food. Soy milk full of essential amino acids is a good nutritional resource for humans; in addition, the rich in isoflavones provide health benefits. However, the Maillard reaction occurs during heating processes or storage because the reactive amino group of the amino acid reacts with the carbonyl group of the sugar, leads to the degradation of amino acids and may generate hazardous substances in food. For instance, furosine, carboxymethyl-lysine (CML) and carboxyethyl-lysine (CEL). In order to holistic understand the quality of soy milk, the non-targeted analysis was performed without any derivatization was developed using high-resolution mass spectrometry (HRMS) to simultaneously analyze the nutrients and hazards compound in soy milk. The comprehensive analysis of soy milk may prove the scientific evidence for the evaluation of half-life of the product. To achieve the goal of this study, three-year projects were points out sequentially. The non-targeted metabolomics study was performed using HRMS to comprehensively analyze the nutrients and hazard components in soy milk. In addition, the stability test was carried out to find out the chemical reaction kinetics of amino acids and the products of Maillard reaction for estimating the half-life of soy milk. The quantitation of hazardous components may useful for risk assessment on intake of soy milk. This study provides a novelty non-targeted metabolomics strategy for investigating the food contaminates, to achieve the quality and safety of food.non-targeted metabolomics; food contaminants; soy milk; amino acids; Maillard reaction; high-resolution mass spectrometry