Yang J.Restori K.H.Xu M.Song E.H.Zhao L.Hu S.Lyu P.WEI-BEI WANGXiong N.2024-09-182024-09-182020-04-24https://www.scopus.com/inward/record.uri?eid=2-s2.0-85082872019&doi=10.1016%2fj.isci.2020.101014&partnerID=40&md5=a8a1912a6bedb31b22d3db63c82fd69ehttps://scholars.lib.ntu.edu.tw/handle/123456789/721254Article Number:101014Proper immune cell development at early ontogenic stages is critical for life-long health. How resident immune cells are established in barrier tissues at neonatal stages to provide early protection is an important but still poorly understood question. We herein report that a developmentally programmed preferential generation of skin-homing group 1 innate lymphoid cells (ILC1s) at perinatal stages helps regulate early skin microbiota colonization. We found that a population of skin-homing NK1.1+ ILC1s was preferentially generated in the perinatal thymi of mice. Unique thymic environments and progenitor cells are responsible for the preferential generation of skin-homing NK1.1+ ILC1s at perinatal stages. In the skin, NK1.1+ ILC1s regulate proper microbiota colonization and control the opportunistic pathogen Pseudomonas aeruginosa in neonatal mice. These findings provide insight into the development and function of tissue-specific immune cells at neonatal stages, a critical temporal window for establishment of local tissue immune homeostasis. © 2020 The Author(s)ImmunologyMicrobiologyjournal article10.1016/j.isci.2020.1010142-s2.0-85082872019