Caffeic acid phenethyl ester inhibits nuclear factor-κB and protein kinase B signalling pathways and induces caspase-3 expression in primary human CD4+ T cells

LI-CHIEH WANGChu K.-H.Liang Y.-C.Lin Y.-L.BOR-LUEN CHIANG2020-12-212020-12-2120100009-9104https://www.scopus.com/inward/record.uri?eid=2-s2.0-77950657523&doi=10.1111%2fj.1365-2249.2009.04067.x&partnerID=40&md5=2b46cd18874a879b3d51db2c9b6164d4https://scholars.lib.ntu.edu.tw/handle/123456789/529668Summary Caffeic acid phenethyl ester (CAPE), an active component in propolis, is known to have anti-tumour, anti-inflammatory and anti-oxidant properties. In this study, the effects of CAPE on the functions of primary human CD4+ T cells were evaluated in vitro. CAPE significantly suppressed interferon (IFN)-γ and interleukin (IL)-5 production and proliferation of CD4+ T cells stimulated by soluble anti-CD3 and anti-CD28 monoclonal antibodies in both healthy subjects and asthmatic patients. CAPE inhibited nuclear factor (NF)-κB activation and protein kinase B (Akt) phosphorylation, but not p38 mitogen-activated protein kinase (MAPK) phosphorylation in T cells. CAPE also induced active caspase-3 expression in CD4+ T cells; CCR4+CD4+ T cells were more sensitive to CAPE induction than CXCR3+CD4+ T cells. Together, these results indicate that CAPE inhibits cytokine production and proliferation of T cells, which might be related to the NF-κB and Akt signalling pathways, and that CCR4+CD4+ T cells are more sensitive to CAPE inhibition. This study provides a new insight into the mechanisms of CAPE for immune regulation and a rationale for the use of propolis for the treatment of allergic disorders. ? 2010 British Society for Immunology.[SDGs]SDG3caffeic acid phenethyl ester; caspase 3; chemokine receptor CCR4; chemokine receptor CXCR3; gamma interferon; I kappa B kinase alpha; I kappa B kinase beta; immunoglobulin enhancer binding protein; interleukin 5; mitogen activated protein kinase p38; protein kinase B; adult; allergic disease; article; asthma; CD4+ T lymphocyte; controlled study; cytokine production; cytokine release; human; human cell; immunoregulation; lymphocyte proliferation; priority journal; protein expression; protein phosphorylation; signal transduction; Antigens, Dermatophagoides; Asthma; Caffeic Acids; Caspase 3; CD4-Positive T-Lymphocytes; Cells, Cultured; Cytokines; Drug Evaluation, Preclinical; Enzyme Activation; Enzyme Induction; Humans; Lymphocyte Activation; NF-kappa B; p38 Mitogen-Activated Protein Kinases; Phenylethyl Alcohol; Phosphorylation; Propolis; Protein Processing, Post-Translational; Proto-Oncogene Proteins c-akt; Receptors, CCR4; Receptors, CXCR3; Signal Transduction; T-Lymphocyte SubsetsCaffeic acid phenethyl ester inhibits nuclear factor-κB and protein kinase B signalling pathways and induces caspase-3 expression in primary human CD4+ T cellsjournal article10.1111/j.1365-2249.2009.04067.x200594792-s2.0-77950657523