楊長豪2006-07-262018-07-122006-07-262018-07-122003http://ntur.lib.ntu.edu.tw//handle/246246/26734The prevention and treatment of myopia are important issue of public health in many countries, especially in Taiwan where the prevalence rate of myopia is extremely high. The most important complication of extreme myopia is retinal degeneration affecting the posterior pole that is associated with elongation of ocular axial length. Unfortunately, the actual mechanism of the development of myopia is still unknown. Isolation and identification of genes expressed in form deprived ocular tissue may aid in the molecular evaluation and understanding of possible mechanisms involved in form deprivation myopia. Subtraction-hybridization PCR method (Fig. 1) is used to selectively amplify target cDNA fragments and simultaneously suppress nontarget DNA amplification. It can achieve greater than 1000-fold enrichment of differentially expressed cDNAs (ie. cDNA from myopic and control eyes). The basic idea of subtraction-hybridization PCR is those tracers DNA (in our experiment, the cDNA from myopic eyes) will primarily reassociate with excess driver DNA (in our experiment, the cDNA from control eyes) while target sequences having no counterparts in driver will inevitably reassociate with each other, or remain single-stranded. The reassociated fragments common for driver and tracer are discarded, and the remaining DNA enriched in target sequences is cloned and analyzed. With this method, Ishibashi et al. showed the upreglation of crystalline mRNAs in form-deprived chick eyes (Exp Eye Res 2000 70:153-158). It is proven that subtraction-hybridization PCR is a powerful tool to study chick myopia. In this experiment, subtraction hybridization technique was used to evaluated the differential gene expression in the chick sclera of myopic and control eyes.application/pdf492628 bytesapplication/pdfzh-TW國立臺灣大學醫學院眼科[SDGs]SDG3journal articlehttp://ntur.lib.ntu.edu.tw/bitstream/246246/26734/1/912314B002406.pdf