Hsiung, L.-C.L.-C.HsiungYang, C.-H.C.-H.YangChiu, C.-L.C.-L.ChiuChen, C.-L.C.-L.ChenWang, Y.Y.WangLee, H.H.LeeCheng, J.-Y.J.-Y.ChengMING-CHIH HOHSIN-YU LEEANDREW WO2020-02-192020-02-1920080956-5663https://www.scopus.com/inward/record.uri?eid=2-s2.0-67549117746&doi=10.1016%2fj.bios.2008.07.027&partnerID=40&md5=34b6aa08d07301fb9faef9a4c66b9988https://scholars.lib.ntu.edu.tw/handle/123456789/461669Uniform patterning of cells is highly desirable for most cellular studies involving cell-cell interactions but is often difficult in an in vitro environment. This paper presents the development of a collagen-coated planar interdigitated ring electrode (PIRE) array utilizing positive dielectrophoresis to pattern cells uniformly. Key features of the PIRE design include: (1) maximizing length along the edges where the localized maximum in the electric field exists; (2) making the inner gap slightly smaller than the outer gap in causing the electric field strength near the center of a PIRE being generally stronger than that near the outer edge of the same PIRE. Results of human hepatocellular carcinoma cells, HepG2, adhered on a 6 × 6 PIRE array show that cells patterned within minutes with good uniformity (48 ± 6 cells per PIRE). Cell viability test revealed healthy patterned cells after 24 h that were still confined to the collagen-coated PIREs. Furthermore, quantification of fluorescence intensity of living cells shows an acceptable reproducibility of cell viability among PIREs (mean normalized intensity per PIRE was 1 ± 0.138). The results suggest that the PIRE array would benefit applications that desire uniform cellular patterning, and improve both response and reproducibility of cell-based biosensors. ? 2008 Elsevier B.V. All rights reserved.[SDGs]SDG3Cell patterning; Cell-based biosensors; Cell-cell interactions; Cell-viability; Cellular microarray; Dielectrophoresis; Electric field strength; Electrode array; Electrode arrays; Fluorescence intensities; Hepatocellular carcinoma; In-vitro; Living cells; Microfluidics; Reproducibility; Biosensors; Cells; Collagen; Electric field effects; Electric fields; Electrolysis; Electromagnetic field theory; Electromagnetic fields; Electrophoresis; Gallium alloys; Metallizing; Cytology; collagen; article; biosensor; cell strain HepG2; cell viability; cytology; electric field; electrode; electrophoresis; fluorescence; human; human cell; liver cell carcinoma; microfluidic analysis; nonhuman; planar interdigitated ring electrode array; quantitative analysis; rat; reproducibility; simulation; uniform cellular patterning; Carcinoma, Hepatocellular; Cell Culture Techniques; Cell Line, Tumor; Cell Separation; Electrophoresis; Equipment Design; Equipment Failure Analysis; Humans; Microelectrodes; Micromanipulationjournal article10.1016/j.bios.2008.07.027187609112-s2.0-67549117746