2008-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/701115摘要：本研究的目的為（1）評估PCV2 對血液單核球所衍生不同分化成熟度的巨噬細胞之影響，（2）探討PCV2 引發肉芽腫性炎症反應的機制，（3）釐清巨噬細胞與淋巴細胞間的交互反應及免疫活化因子在PCV2 引發肉芽腫性炎症反應上所扮演的角色。；計畫中擬以活體外方式，經由豬隻周邊血液單核球所衍生之不同分化成熟度的巨噬細胞(monocyte-derived macrophages, MDMs)之誘發、定性及培養技術之建立，再於單純PCV2 接種或同時混合 有淋巴細胞及/或細菌內毒素（LPS）或T 淋巴細胞致裂原concannavalin A 等免疫活化因子培養條件下，分析相關免疫細胞的PCV2感受性、細胞存活率、細胞總數、多核巨細胞的形成、抗原呈獻、趨化能力、吞噬力、殺菌力、細胞凋零率、細胞毒殺能力，以及各類炎症反應中介質及細胞激素的產生能力等。<br> Abstract: The objectives of the present study are (1) to evaluate the effects of PCV2 on blood monocyte-derived macrophages (MDMs) of different maturation stages, (2) to elucidate the pathogenesis of PCV2-induced granulomatous inflammation, and (3) to determine the role of macrophage/lymphocyte interaction and immune-activating factors on the development of PCV2-induced granulomatous inflammation. MDMs of different maturation stages will be generated, characterized, and inoculated with PCV2 with or without co-culture with lymphocytes and/or lipopolysaccharide and concanavalin A in vitro. Changes in the susceptibility of the associated immune cells to PCV2, cell viability, total cell count, formation of multinucleated giant cells, functions of antigen presenting, chemotaxis, phagocytosis, microbe killing, apoptic rate, cytotoxicity, and inflammatory mediator/cytokine production etc. will be evaluated.豬第二型環狀病毒肉芽腫性炎症反應豬Porcine circovirus type 2 (PCV2)granulomatous inflammationpig