WEI-WEN LIUChen, Sheng HongSheng HongChenPAI-CHI LI2023-05-052023-05-052021-01-012156-7085https://scholars.lib.ntu.edu.tw/handle/123456789/630860This study demonstrates that chlorophosphonazo III (CPZ III) can be used as a contrast agent for photoacoustic calcium imaging. CPZ III can pass across the plasma membrane for labeling intracellular Ca2+ without cytotoxicity. In optical-resolution photoacoustic microscopy (OR-PAM), the photoacoustic (PA) signal intensity was strongly correlated with the presence of CPZ III and Ca2+ at various concentrations. The sensitivity of PA signal reception was enhanced by using an 8 MHz single-element focused ultrasound detector due to their matched frequency characteristics. Differences in the PA signal intensity were successfully found between the core and margin areas of tumorspheres in three-dimensional cell cultures. These findings indicate that CPZ III can serve as a novel PA contrast agent for functional Ca2+ imaging using OR-PAM.enMODULATES GENE-EXPRESSION; NEURONAL-ACTIVITY; RECEPTORS; TARGETjournal article10.1364/BOE.414602336805642-s2.0-85102073278WOS:000616007100004https://api.elsevier.com/content/abstract/scopus_id/85102073278