2006-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/664890摘要：為提高辣椒一代雜種種子的成本效益，本計畫擬利用DNA連鎖複製的方法(PCR-based methods)配合BSA（bulk segregant analysis）使用，尋找雄不稔性基因的分子標誌。植物材料為七個互為自交五代以上的近自交系(near-isogenic lines) 辣椒，其中四個為稔性系統；雄不稔系統後代分離比為可稔：不稔為3:1；稔性系統的後代全為可稔，顯示本系統的雄不稔性為單一隱性核基因所控制。本年度的目標在找出此雄不稔性基因的分子標誌，及評估其適用性。<br> Abstract: The objective of this study is to identify molecular marker tightly linked to male sterility in hot pepper, using PCR-based method along with BSA（Bulk Segregant Analysis）.The 7 near-isogenic lines were selfed for at least 5 generations, and 3 of them are male sterile. The male sterility is this system is determined by one recessive genomic gene, given a 3:1 ratio from a heterozygote. In this present year, the project would be first focused on the screening and identification of molecular linked to the gene affected male sterility in this system. Any identified markers will be used to evaluate the likage between the marker and male sterility among other lines.辣椒雄不稔性一代雜種分子標誌hot peppermale sterilityF1 hybridmolecular marker