2013-08-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/658617摘要：Wnt信號路徑過去被認為在組織和器官的發育及維持扮演相當重要的角色。近年來，Wnt信號家族的基因也陸續在線蟲、果蠅以及脊椎動物被發現及分析。然而，Wnt信號傳導路徑在病媒蚊體內的功能幾乎一無所知。若能詳加研究病媒蚊之生殖及發育調控的機制，相信將會對未來制訂蚊媒疾病控制替代性的策略有相當助益。基於過去的研究報導以及我們初步的研究結果，我們推測Wnt信號路徑在調控病媒蚊的生殖及發育，扮演相當重要的角色。在這三年期的研究計畫中，我們將詳盡的探討Wnt信號路徑如何在登革熱的重要病媒蚊埃及斑蚊中，調控其生殖及發育之過程。第一年的計畫中，我們將探討Wnt信號路徑中的穿膜蛋白Frizzleds在調控病媒蚊生殖過程的功能。第二年我們將探討不同的Wnts在調控病媒蚊生殖過程中所扮演的角色，以及其與TOR信號路徑的交互作用。第三年的研究，我們將探討Wnts在病媒蚊發育過程中所參與的調控機制。在本研究結束前，我們應該能清楚的勾勒出Wnt信號路徑參與生殖調控及發育過程的網絡。本研究的結果將有助於我們對Wnt信號路徑在不同物種演化保留性的全面性瞭解，並且對於未來制訂病媒控制策略將有相當大的幫助。<br> Abstract: Wnt signaling pathway is an important aspect of tissue/organ development and maintenance. Members of Wnt signaling have been identified and characterized from nematode to Drosophila and vertebrates. However, the roles of Wnt signaling in the mosquito remain largely unknown. Intensive study for the reproduction and development in the mosquito vector will benefit the establishment of alternative strategy to combat mosquito-borne diseases. Based on previous studies and our preliminary results, we speculate that Wnt signaling pathway plays crucial roles in the regulation of mosquito reproduction and development. In this 3-year proposal, we will dissect the detailed regulation machinery of Wnt signaling in the fecundity and morphogenesis in the mosquito Aedes aegypti, the major vector for dengue virus. In the first year, we will investigate the function of Aedes aegypti Frizzleds, the transmembrane Wnt receptors, in the regulation of mosquito reproduction. In the second year, we will investigate the roles of Aedes aegypti Wnts in the mosquito egg production and the crosstalk between TOR signaling pathway. In the third year, we will investigate the roles of Aedes aegypti Wnts in the development of mosquito. By the end of this study, we should be able to illustrate a detail network of Wnt signaling pathway in the regulation of mosquito reproduction and morphogenesis. Detail characterization of mosquito Wnt signaling in this proposal will provide new insights into the evolutionary conserved features of Wnt signaling and will pave the way toward the establishment of efficient strategies for vector control.埃及斑蚊生殖發育信號路徑病媒控制Aedes aegyptireproductiondevelopmentsignaling pathwayvector control