2012-08-202024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/683813摘要：土肉桂(Cinnamomoum osmophloeum Kaneh.)為樟科(Lauraceae)樟屬(Cinnamomum)之台灣特有闊葉樹種。至目前為止，已有許多的研究指出土肉桂之二次代謝產物(抽出成分)對於細菌、黴菌、白蟻及&#34728;等微生物或昆蟲具有不錯的抑制效果。然而，土肉桂具有多樣的化學品系，因此如何區分不同的化學品系從事有效的生產則成為當務之急知課題，但就分子生物學的角度來看，這些參與土肉桂生長、發育及二次代謝物的生合成相關酵素研究，則尚未有研究論文發表。本計畫將選殖肉桂醛和枷羅木醇生合成的相關基因，包括phenylalanine ammonia-lyase (PAL)、trans-cinnamate-4- hydroxylase (C4H)、4-coumarate:CoA ligase 1 (4CL1)、4-coumarate:CoA ligase 3 (4CL3)、cinnamoyl-CoA reductase (CCR)和Linalool synthase (LIS)，並利用反轉錄聚合&#37238;連鎖反應(reverse transcription-polymerase chain reaction, RT-PCR)，進行進行不同品系差異基因之表現圖譜。此外，所選殖得到之 基因將透過蛋白質表現系統進行基因功能鑑定。<br> Abstract: Cinnamomum osmophloeum Kaneh. belongs to the Cinnamomum genus of the Lauraceae family is an economically important tree that is endemic species of Taiwan. There are several papers dealing with the antibacteria, antifungal, antitermite, antiant, and antitumor activities of the extractives isolated from C. osmophloeum. However, there are numerous chemotypes with various secondary metabolite profiles in C. osmophloem. Thus, it becomes important topic to distinguish different chemotype for effective production. However, investigation of the importance of enzymes in the plant development has been hampered by a lack of information at a molecular level. Therefore, in this proposed project, we will study on the functional genomics of Cinnamomum osmophloeum. In this project, we will focus on the relative genes involved in cinnamaldehyde and linalool biosynthesis, including phenylalanine ammonia-lyase (PAL)、trans-cinnamate-4-hydroxylase (C4H)、4-coumarate:CoA ligase 1 (4CL1)、4-coumarate:CoA ligase 3 (4CL3)、cinnamoyl-CoA reductase (CCR) and linalool synthase (LIS). In order for compared the difference provenances, reverse transcription-polymerase chain reaction (RT-PCR) was use for analysis the genes expression pattern. In addition, the isolated genes will be confirmed by recombinant protein expression system for the functional characterization.土肉桂肉桂醛枷羅木醇Cinnamomum osmophloeumcinnamaldehydelinalool