Lee H.-W.Deng M.-C.Pan C.-H.HUI-WEN CHANGCheng I.-C.2020-01-062020-01-06201803781135https://scholars.lib.ntu.edu.tw/handle/123456789/446067In the case of serotype O foot-and-mouth disease virus (FMDV), antibodies against five neutralizing sites play a pivotal role in protection of animals, with site 1 being considered the most crucial. However, recent studies indicated that the antibodies of vaccinated farm animals are mainly against site 2 rather than site 1. In Taiwan, blanket vaccination had been implemented for more than fifteen years, in which the porcinophilic isolate O/Penghu/2012 showed significant amino acid alterations in site 2 compared to the early isolate O/TW/97. To study the antigenicity of site 2, MAbs against site 2 are required. In this study, we generated site 2 mutated virus-like particles (mVLPs) with only VP2-S72 N mutation, and successfully identified five site 2 MAbs from a previously prepared O/TW/97 MAb panel by immunofluorescence assay (IFA) and ELISA based on the different reactivity to wild-type VLP and mVLP. In conclusion, the established model was proved as an effective method to reveal the epitope that a MAb recognizes. By applying this MAb panel and sequence alignment, we demonstrated that the O/Penghu/2012 isolate not only showed significant genetic difference in site 2 but also significant antigenic difference from the ancestral O/TW/97. ? 2018 Elsevier B.V.Foot-and-mouth disease virus; Monoclonal antibody; Mutagenic; Neutralizing; Virus-Like particle[SDGs]SDG3monoclonal antibody; neutralizing antibody; epitope; monoclonal antibody; neutralizing antibody; virus antibody; virus antigen; animal cell; antigenicity; Article; controlled study; enzyme linked immunosorbent assay; Foot and mouth disease virus; immunofluorescence test; mouse; nonhuman; virus like agent; virus mutation; animal; foot and mouth disease; Foot and mouth disease virus; genetics; immunology; mutation; pig; serodiagnosis; swine disease; Taiwan; virology; Animals; Antibodies, Monoclonal; Antibodies, Neutralizing; Antibodies, Viral; Antigens, Viral; Epitopes; Foot-and-Mouth Disease; Foot-and-Mouth Disease Virus; Mutation; Neutralization Tests; Swine; Swine Diseases; Taiwanjournal article10.1016/j.vetmic.2018.06.0022-s2.0-85050112719https://www2.scopus.com/inward/record.uri?eid=2-s2.0-85050112719&doi=10.1016%2fj.vetmic.2018.06.002&partnerID=40&md5=de3e6de0a4bb98c21bbb121a3ed69413