2017-01-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/643113摘要：漿狀樹突細胞(pDC)是一群特別的免疫細胞，可以再發炎和感染的狀態下產生大量的第一型干擾素(IFＮ-I)。我們之前的研究顯示，經由FL刺激CLP (common lymphoid progenitor)產生的IFN-I可以促進CLP表面Flt3的表現，增加CLP存活率和分裂並分化成pDC的能力。因此FL偕同IFN-I可以調控CLP分化成pDC。此外，CLP表現許多TLR，例如TLR2, TLR4, TLR7和TLR9。體外刺激不同的TLR配體或感染流感病毒會透過抑制pDC生成，並促進cDC生成而改變FL誘導CLP分化成DC的過程，這意味發炎或感染的狀態可以影響DC恆定。 在這個計畫，我們要回大兩個重要問題，第一個是高劑量和低劑量的流感病毒感染對於DC分化和功能的調控。 第二個是，IFN-I和M-CSF對於DC恆定性的影響。<br> Abstract: Plasmacytoid DC (pDCs) are specialized immune cells that are capable of producing large quantities of type I interferon (IFN-I) during inflammation and infection. We have previously shown that IFN-I was induced by FL in common lymphoid progenitors (CLPs), facilitating the up-regulation of Flt3 expression and enhancing survival and proliferation of CLPs and their differentiation into pDCs. Therefore, FL coordinates with IFN-I to regulate pDC development from CLPs. Moreover, CLPs express various TLRs, including TLR2, TLR4, TLR7 and TLR9. The administration of TLR agonists or infection of influenza virus in vitro altered FL-dependent DC development by inhibiting pDC but promoting cDC generation from CLPs, suggesting that inflammation and infection may modulate DC homeostasis. In this proposal we aim to address two important issues. First, to study the effect of high or low virulent influenza virus on DC development and function during infection. Second, to study the effects of IFN-I and M-CSF on DC homeostasis.樹突細胞干擾素流感病毒dendritic cellIFNinfluenza