2012-08-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/661048摘要:amiRNA-介導的交叉保護性,是另一種植物的防禦反應,以保護之前感染相同的病毒弱毒疫苗株病毒感染的植物。我們已經確定了3個保守的氨基酸(R180I,F205L,E396N),HC-Pro的病毒抑制,導致寄主植物上的症狀輕微。嚴重和輕度病毒(林艾拉,2007年)之間的相互作用研究,我們開發了一個單位的制度。在這項工作之後,我們發現,HC-Pro的miRNA介導的基因調控抑制相關症狀的發展。我們發現3個保守的HC-Pro的氨基酸(R180I,F205L,E396N)是必不可少的miRNA途徑抑制,但不影響siRNA的途徑,說明HC-Pro的抑制miRNA途徑與siRNA途徑是不同的。<br> Abstract: The amiRNA-mediated resistance, cross-protection is another plant defense response to protect a plant from virus infection by prior infection with an attenuated strain of the same virus. We have identified 3 conserved amino acids (R180I, F205L, E396N) on the viral suppressor HC-Pro that causes the mild symptoms on the host plant. We developed a unit system for studying the interaction between the severe and mild viruses (Lin el al., 2007). Following this work, we demonstrated that the symptoms development is correlated with inhibition of miRNA-mediated gene regulation by HC-Pro. We found the 3 conserved amino acids (R180I, F205L, E396N) on the HC-Pro are essential for miRNA pathway suppression, but did not affect the siRNA pathway, indicating the HC-Pro suppresses the miRNA pathway is different with siRNA pathway (Wu et al., 2010).以系統生物學策略探討植物病源逆境機制及發展PTGS相關小分子核酸分析平台The study of plant pathogen stresses by systems biology and developing the analysis platform of the PTGS-related small RNA前瞻研究領航計畫-以系統生物學策略探討植物病源逆境機制及發展PTGS相關小分子核酸分析平台