M. Y. ChouJ. C. HungL. C. WuS. P. L. HwangP. P. Hwang2019-07-242019-07-2420111420 682Xhttps://scholars.lib.ntu.edu.tw/handle/123456789/414722https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960933698&doi=10.1007%2fs00018-010-0593-2&partnerID=40&md5=3bf74db40a975f4225ecbab87797d78bThe present study using zebrafish as a model explores the role of isotocin, a homolog of oxytocin, in controlling ion regulatory mechanisms. Double-deionized water treatment for 24 h significantly stimulated isotocin mRNA expression in zebrafish embryos. Whole-body Cl-, Ca2+, and Na+ contents, mRNA expressions of ion transporters and ionocyte-differentiation related transcription factors, and the number of skin ionocytes decreased in isotocin morphants. In contrast, overexpression of isotocin caused an increase in ionocyte numbers. Isotocin morpholino caused significant suppression of foxi3a mRNA expression, while isotocin cRNA stimulated foxi3a mRNA expressions at the tail-bud stage of zebrafish embryos. The density of P63 (an epidermal stem cell marker)-positive cells was downregulated by isotocin morpholinos and was upregulated by isotocin cRNA. Taken together, isotocin stimulates the proliferation of epidermal stem cells and differentiation of ionocyte progenitors by regulating the P63 and Foxi3a transcription factors, consequently enhancing the functional activities of ionocytes. © The Author(s) 2010.Differentiation; Ion; Ionocyte; Isotocin; Zebrafishisotocin; protein p63; animal tissue; article; cell density; cell differentiation; cell proliferation; controlled study; down regulation; embryo; foxi3a gene; gene; gene overexpression; gene repression; immunohistochemistry; ionocyte; nonhuman; nucleotide sequence; osmosis; plasmid; sequence homology; skin cell; stem cell; Animals; Cell Differentiation; Cell Proliferation; Down-Regulation; Embryo, Nonmammalian; Forkhead Transcription Factors; Ion Transport; Ions; Oxytocin; Phosphoproteins; Stem Cells; Trans-Activators; Zebrafish; Zebrafish Proteins; Danio rerioIsotocin controls ion regulation through regulating ionocyte progenitor differentiation and proliferationjournal article10.1007/s00018 010 0593 2211042922-s2.0-79960933698