林輝2006-08-232018-06-292006-08-232018-06-291999-07-31http://ntur.lib.ntu.edu.tw//handle/246246/29524A novel method of preparation of easily stripped off temporary wound dressing material is disclosed. A tri-layer membrane system for artificial skin is prepared in this study. In this process, the N-isopropyl acrylamide monomer is successfully grafted on the non-woven fabric by copolymerization. It is initiated by plasma to activate the surface of the non-woven cloth. N-isopropyl acrylamide is then grafted onto the surface of the non-woven cloth by -ray irradiation. The last layer but the most important of a bovine gelatin with glycosaminoglycans (chondroitin-6-sulfate) is grafted by UV light, which serves as a matrix for the infiltration of fibroblasts, macrophages, lymphocytes, and capillaries derived from the wound bed. The goal in this work is to provide such a nonantigenic membrane closely resembling dermis in its anatomic structure and chemical composition, which would act as a scaffolding inducing the synthesis of a new dermis. The following describes the construction and animal testing of this artificial skin in extensively damage. In the experiment, the specimen are divided into 4 groups: (1) controlled group without dressing material, (2) non-woven fabric, (3) non-woven fabric grafted with NIPAAm, and (4) non-woven fabric grafted with NIPAAm, bovine gelatin, and glycosaminoglycans from bottom to top in sequence. After operated for 6 weeks, both controlled group and non-woven fabric group stayed in the proliferative phase where no epidermis or dermis structure has been traced in the section. 6 weeks of postoperation, the third group has been healed completely in the maturation phase. The wound site has been totally recovery with normal dermis and epidermis structure around but the dressing material still stayed on the wound site. In the group of the non-woven fabric grafted with NIPAAm, geltain, and glycosaminoglycans, it has been recovery to the final stage of maturation phase. The wound site has been totally recovery at the 4th week of postoperation. The dressing material of the group fall off automatically from the wound site without any damage to the skin after recovery. We believe the dressing material will have a great potential in medical application in the near futureapplication/pdf28952 bytesapplication/pdfzh-TW國立臺灣大學醫學工程學研究所reporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/29524/1/883011B002003NU.pdf