Wang H.-T.Yang L.-C.Yu H.-C.Chen M.-L.Wang H.-J.HUEI WANG2019-07-162019-07-16201810219498https://scholars.lib.ntu.edu.tw/handle/123456789/414024Fucose is one of important residues of recognition pattern for many immune cells. In this study, we characterized bioactive fucose-containing acidic polysaccharides from submerged fermentation of Agaricus blazei Murill. We obtained the polysaccharides through a cell-based activity-guided strategy, and used carbohydrate recognition monoclonal antibodies based Enzyme-Linked Immuno Sorbent Assay (ELISA) along with methylation and NMR analyses to investigate the structural characteristics of the polysaccharides. The polysaccharides had Mw of 3.5 ¡Ñ 10 5 Da. The major sugars were L-fucose, L-arabinose, D-galactose, D-xylose, and D-galacturonic acid in the molar ratio of 6.4, 15.5, 28.5, 14.7, and 25.0% with a small amount of D-glucose, D-mannose, L-rhamnose, and D-glucuronic acid. Results indicated that the bioactive polysaccharides consisted of a (1,4)-Galp and (1,4)-GalAp back bone; (1,2)-Xyl and (1,2)-Rha might also comprise backbone or constitute side chain; linkage (1,5)-Ara and terminal fucosyl residues were also involved in the polysaccharides. Regarding bioactivity, removal of the terminal L-fucosyl residues reduced the TNF-£\ cytokine stimulating activity of the polysaccharides in a RAW 264.7 macrophage cell-line test, whereas NF-£eB and TLR4 affected the polysaccharide-induced TNF-£\ production. ? 2017Acidic polysaccharideAgaricus blazei MurillFucoseGalactanTNF-£\ stimulation[SDGs]SDG3journal article10.1016/j.jfda.2017.07.006https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028672022&doi=10.1016%2fj.jfda.2017.07.006&partnerID=40&md5=b03f1594ab3b9ccc4277931d962e7eb52-s2.0-85028672022https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028672022&doi=10.1016%2fj.jfda.2017.07.006&partnerID=40&md5=b03f1594ab3b9ccc4277931d962e7eb5