李嘉哲2006-08-312018-07-112006-08-312018-07-112000http://ntur.lib.ntu.edu.tw//handle/246246/29768D 型肝炎病毒有一些獨特的生物機能 例如自我催化之切割及連結的活性、RNA 編輯活性、與D 型肝炎抗原相關之病毒複 製。這些活性應有其它細胞因子與D 型肝 炎病毒RNA 或抗原交互作用而產生。噬菌 體呈現法利用噬菌體將外來蛋白表現在其 表面蛋白上。它是研究蛋白間交互作用或 蛋白及核酸交互作用很有用的工具之一。 我們用T７ 噬菌體製造了一個人肝對補去 氧核甘酸的基因庫。利用biotin 標記的D 型肝炎病毒RNA 探針來篩檢基因庫，我們 得到了一些可能作用的基因。這包括人類 白蛋白，一leucine zipper 蛋白， apolipoprotein E ，phosphodiesterase 3B ，及一些粒腺體中的蛋白。其中只有白蛋 白通過in vitro 結合試驗。我們正在研究 白蛋白與D 型肝炎病毒RNA 交互作用是否有生物學上的意義。Hepatitis delta virus (HDV)possesse s several distinct biological activities such as autocatalytic cleavage and ligation activity,RNA-editing activity,hepatitis delta an tigen (HDAg)dependent replication transac tivation and inhibition. There should be some cellular factor s interacting with HDV RNA or HDAg to accomplish these activities. The phage display technique utilizes phages to express foreign proteins o r peptides in fusion with one of their coat protein.It is an ideal tool to study protein-protein interaction and protein-nucleic acid interaction . We constructed a T7 phage expression CDNA library f rom a human liver and selected with biotin-labeled genomic HDV RNA probe.We obtained several candidate genes including human albumin,a leucine zipper protein, apolipoprotein E,phosphodiesterase 3B and some mitochondria-related proteins. Only human albumin stood the in vitro binding test including northwestern and gel mobility shift assay.We are currently investigatin g the biological meaning of this HDV RNA and human albumin interaction.application/pdf24803 bytesapplication/pdfzh-TW國立臺灣大學醫學院內科D 型肝炎病毒噬菌體呈現法Hepatitis delta virusphage display[SDGs]SDG3reporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/29768/1/892315B002016MH.pdf