生物資源暨農學院: 植物醫學碩士學位學程指導教授: 葉信宏; 洪挺軒陳盈如Chen, Ying-JuYing-JuChen2017-03-032018-06-292017-03-032018-06-292016http://ntur.lib.ntu.edu.tw//handle/246246/275005胡瓜嵌紋病毒 (Cucumber mosaic virus, CMV)在世界上分布範圍廣泛，寄主繁多，目前已知1200種以上，跨越100多科之植物皆為其寄主，加之CMV在田間可被75種以上之蚜蟲傳播，若缺乏適當之防治，易導致產量減少造成經濟上損失，為經濟上極為重要之植物病毒，然而目前對CMV所引起之病害仍然缺乏長期、有效之防治方法。本研究擬利用交叉保護以及土壤微生物分泌之代謝物誘發植物產生抗性，來對CMV所引起之病害進行防治。在交叉保護上，利用接種已截切掉CMV 2b蛋白之弱系CMV於圓葉菸草上，已證實此弱系之CMV可以交叉保護多種強系之CMV。在利用土壤微生物分泌之代謝物誘發植物產生抗性上，發現自土壤中分離之菌株NTU1F8培養後之過濾液，於圓葉菸草上可以有效抑制胡瓜嵌紋病毒之感染以及病程之發展。此外，於圓葉菸草及芥菜上，培養濾液亦可抑制蕪菁嵌紋病毒之感染。Cucumber mosaic virus (CMV) is an economically important plant viruses with a worldwide distribution and a very wide host range. We aim to use two strategies to manage disease caused by CMV on crops. One is to develop cross protection by utilization of mild strain virus and the other is to induce plant resistance in plant by treatment of culture filtrate derived from fungi (NTU1F8) isolated from soil. We first conducted our strategies on Nicotiana benthamiana. The result showed that 2b gene-partial deleted CMV induced only mild symptom on N. benthamiana and protect plant from infection by different severe strains of CMV. In addition, the treatment of culture filtrate of NTU1F8, can obviously decrease infection rate, delay symptom development and decrease virus accumulation. We also found pretreatment of N. benthamiana and Brassica juncea with culture filtrate of NTU1F8 following inoculation of Turnip mosaic virus could also decrease the infection rate and disease severity.論文使用權限: 不同意授權2b 蛋白胡瓜嵌紋病毒誘導性植物抗性弱系病毒土壤微生物蕪菁嵌紋病毒2b proteinCucumber mosaic virusinduced plant resistancemild strain virussoil microorganismsTurnip mosaic virusthesis10.6342/NTU201600616