Chen C.-H.Liao C.-H.Chang Y.-L.JIH-HWA GUHPan S.-L.Teng C.-M.2021-06-022021-06-0220123043835https://www.scopus.com/inward/record.uri?eid=2-s2.0-81955167334&doi=10.1016%2fj.canlet.2011.09.042&partnerID=40&md5=e4094ad88f82febd0eca237b2e53a4aehttps://scholars.lib.ntu.edu.tw/handle/123456789/564808In this study, we investigated the anticancer effect of protopine on human hormone-refractory prostate cancer (HRPC) cells. Protopine exhibited an anti-proliferative effect by induction of tubulin polymerization and mitotic arrest, which ultimately led to apoptotic cell death. The data suggest that protopine increased the activity of cyclin-dependent kinase 1 (Cdk1)/cyclin B1 complex and that contributed to cell apoptosis by modulating mitochondria-mediated signaling pathways, such as Bcl-2 phosphorylation and Mcl-1 down-regulation. In conclusion, the data suggest that protopine is a novel microtubule stabilizer with anticancer activity in HRPC cells through apoptotic pathway by modulating Cdk1 activity and Bcl-2 family of proteins. ? 2011 Elsevier Ireland Ltd.[SDGs]SDG3cyclin B1; cyclin dependent kinase 1; protein bcl 2; protein mcl 1; protopine; antineoplastic activity; apoptosis; article; cancer cell culture; cell cycle arrest; cell cycle G2 phase; cell cycle M phase; cell cycle progression; cell cycle regulation; cell proliferation; concentration response; controlled study; down regulation; drug cytotoxicity; drug structure; drug synthesis; enzyme activation; enzyme activity; human; human cell; in vitro study; intracellular signaling; microtubule assembly; mitochondrion; priority journal; prostate cancer; protein expression; protein phosphorylation; Apoptosis; Benzophenanthridines; Berberine Alkaloids; Cell Growth Processes; Cell Line, Tumor; Humans; Male; Mitosis; Neoplasms, Hormone-Dependent; Prostatic Neoplasms; Signal Transduction; Transfection; Tubulin Modulatorsjournal article10.1016/j.canlet.2011.09.042220332452-s2.0-81955167334