吳兩新2006-07-262018-06-292006-07-262018-06-292001http://ntur.lib.ntu.edu.tw//handle/246246/16443本研究係選用台灣山羊，以腹正中線剖腹術摘取卵巢，利用解剖顯微鏡摘取1-3 mm大小之濾泡，利用酵素解離法將濾泡解離成 單離濾泡細胞，來探討性腺內泌素，對其孕酮(progesterone, P4)分泌及類固醇生成酵素之影響。初步結果顯示：在動情素方面，添加雌二醇(estradiol, E2) 0.1、1 及10ug/ml，無論是基礎與oLH(10 ng)刺激組，均顯著減少濾泡細胞P4 之分泌量;且具有抑制P450 側鏈截切酵素的表現。在雄性素方面，添加睪固酮(testosterone, T)0.1、1ug/ml，無論是基礎與oLH(10 ng)刺激組，均可抑制P4 之分泌。The purpose of this study was to examine the effect of estradiol and testosterone on progesterone production from caprine follicle cells in vitro culture system. The data demonstrated that estradiol could act directly on follicle cell to reduce the secretion of progesterone and expression of P450scc enzyme, and testosterone also could decreases progesterone production in basal and oLH-treated groups. This results implied that the interaction of estradiol and testosterone may play important roles in regulating the steroidogenesis of follicle cell, thus, determining the life span of the follicle.application/pdf29614 bytesapplication/pdfzh-TW國立臺灣大學動物科學技術學系暨研究所山羊濾泡細胞孕酮類固醇生成酵素goatfollicle cellprogesteronesteroidogenesis enzymereporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/16443/1/892313B002181.pdf