2011-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/678737摘要：食蛇龜（Cuora flavomarginata）為台灣原生種烏龜，且為台灣四種原生龜中唯一陸棲型淡水龜，且由於長年棲地的破壞以及走私捕捉，面臨瀕臨絕種的危機，但現今爬蟲類文獻中對於食蛇龜的研究仍相當缺乏，尤其基礎生理及血液學資料皆尚未建立，因此本研究目的在於研究並建立台灣原生食蛇龜於原棲地及圈養下之正常健康體態與血液生化參考值。藉由比較野生食蛇龜於原棲地之血液學及血液生化學參考值與圈養食蛇龜之差異，提供棲地保育及圈養復育下動物健康及環境健康之參考資料。 本實驗研究食蛇龜以圈養及野生食蛇龜作為觀測對象，健康體態參考指標以Jackson’s ratio、體重除以背甲長的對數回歸方程式進行分析。同時建立了食蛇龜的體重背甲長回歸方程式，可根據季節和性別的不同用來預測正常的體重範圍。 在血液生化參考值之建立上，於1月、4月、7月、10月為台灣四季代表月份，以物理保定方式進行頸靜脈抽血，檢驗血液中項目包含血容比、血紅素、紅白血球計數及血球細胞染色。血液生化學包含總蛋白、白蛋白、尿酸、尿素氮、肌胺酸酐、葡萄糖、天門冬酸鹽轉氨&#37238;、氨基丙酸轉氨&#37238;、膽固醇、三酸甘油脂、鹼磷&#37238;、血鈣、血磷、肌酸磷激脢共14項，以國際臨床病理聯盟（International Federation of Clinical Chemistry, IFCC）法則建立正常參考值的範圍，並分析性別及季節單獨對於血液生化值的影響和性別與季節的交互作用。 本實驗所完成的健康體態參考指標和血液生化參考值，可直接應用於獸醫臨床和生態保育相關工作上，將建立之體態與血液生化參考值應用於做為食蛇龜在野生動物救傷、疾病診斷、檢疫等相關工作，評估其健康狀態與提高治療成功率。此外,提供此參考值，做為原生食蛇龜之健康資料，可用於長期監測野外族群及生態及棲地健康，並可用於偵測新興與重要野生動物傳染病，以避免野生族群遞減的危機。 <br> Abstract: Yellow-margined box turtle (Cuora flavomarginata) is the only largely terrestrial geoemydide turtle species in Taiwan. Because of habitat destruction and smuggling, yellow-margined box turtle is endangered in the island. So far scientific research are lacking especially in basic physiology and hematological information. For this purpose, the objective of this study are establishing normal body ratio, hematological and biochemical reference values of captive and wild yellow-margined box turtle. The results will be applied to clinical veterinary medicine and wildlife conservation. Captive and wild yellow-margined box turtles were used in this study. Clinical physical examinations were normal in all turtles. Jackson’s ratio and the equation of logarithm body weight regressed against logarithm carapace length were adopted to assess the health condition. In hematological and biochemical reference values establishment, blood samples were obtained from jugular vein in January, April, July, and October. Complete blood count (CBC) was performed and included PCV, Hb, RBC count, WBC count and WBC differential count. Biochemistry analysis included albumin, total protein, uric acid, urea nitrogen (BUN), creatinine, glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), Alkaline phosphatase (ALKP), cholesterol, triglycerides, calcium, phosphorus and creatine kinase (CK). The principle from International Federation of Clinical Chemistry (IFCC) was utilized for reference range. Data were also analyzed on the basis of sex, season and interaction between sex and season, using repeated measures analysis of variance. The results present in this study can contribute to clinical veterinary medicine and wildlife conservation directly. The results and methods will become a reference for future research.食蛇龜血液學血清生化學體態比例Yellow margined box turtlehematologyserum biochemistrybody ratio