Lin, W.-Y.W.-Y.LinSu, J.-H.J.-H.SuLu, Y.Y.LuWen, Z.-H.Z.-H.WenCHANG-FENG DAIKuo, Y.-H.Y.-H.KuoSheu, J.-H.J.-H.Sheu2018-09-102018-09-102010http://www.scopus.com/inward/record.url?eid=2-s2.0-77249140954&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/356868Five new cembranoids, sarcocrassocolides A-E (1-5), along with three known cembranoids 6-8, have been isolated from a Formosan soft coral Sarcophyton crassocaule. The structures of the new metabolites were elucidated by extensive spectroscopic analysis and the absolute configuration of 1 was determined by a modified Mosher's method. Compounds 1-4 exhibited significantly cytotoxic activity against a limited panel of cancer cell lines. Compounds 1-4, 6 and 8 were shown to exert significant in vitro anti-inflammatory activity in LPS-stimulated RAW264.7 macrophage cells. Compound 6 also significantly inhibited the accumulation of pro-inflammatory COX-2 protein. ? 2010 Elsevier Ltd. All rights reserved.Cembranoids; Inflammatory; Sarcocrassocolides; Sarcophyton crassocaule; Soft coral[SDGs]SDG3[SDGs]SDG14cembranoid; cyclooxygenase 2 inhibitor; inducible nitric oxide synthase; sarcocrassocolide a; sarcocrassocolide b; sarcocrassocolide c; sarcocrassocolide d; sarcocrassocolide e; unclassified drug; animal cell; antiinflammatory activity; article; carbon nuclear magnetic resonance; controlled study; coral; cytotoxicity; drug activity; drug isolation; drug structure; heteronuclear multiple bond correlation; human; human cell; nonhuman; nuclear Overhauser effect; protein expression; proton nuclear magnetic resonance; rat; Sarcophyton crassocaule; spectroscopy; Animals; Anthozoa; Anti-Inflammatory Agents; Cell Line, Tumor; Cyclooxygenase 2; Diterpenes; Drug Screening Assays, Antitumor; Humans; Macrophages; Magnetic Resonance Spectroscopy; Mice; Molecular Conformation; Alcyonacea; Sarcophyton crassocaulejournal article10.1016/j.bmc.2010.01.0362-s2.0-77249140954WOS:000274957100021