2007-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/684554摘要：非生物性逆境往往限制水稻之生長發育並影響稻米之質量及產量。由於水稻之非生物性逆境耐受性之性狀多為數量遺傳基因所控制，傳統之育種改良進展較慢且有極限。隨箸後基因體世代的來臨，功能性基因體學如T-DNA 插入所產生之水稻突變系成為研究水稻不同基因其生理功能與品質、農藝性狀、產量等之重要工具。本計畫為利用水稻T-DNA插入突變系進行發育與抗逆境功能性基因體研究子計畫之一，過去我們已配合其它子計畫進行水稻抗逆境相關突變系於逆境下之GUS染色分析、同型結合突變株之分子鑑定及篩選等。同時亦建立相關農藝性狀及生理、生化等分析條件。本年度之計畫除延伸既有抗逆境相關突變株之篩選外，主要將針對8817 (heat shock protein 83), 27407 (CPEP phosphonomutase), 28360 (LM heat shock protein), 29006 (UDP-glucose 4-epimerase), 34229 (WRKY43)及48000(cytochrome P450)等，於不同非生物逆境下之基因表現、農藝性狀及生理功能加以分析，以增加吾人對水稻非生物逆境耐受機制之瞭解及新育品種之參考。<br> Abstract: Abiotic stress is known as a major factor that limits crop growth and development and affects crop production and quality. Most of abiotic stress tolerance traits in crops are controlled by quantitative trait loci (QTL). The improvement of crop abiotic stress tolerance by conventional breeding or cultivation is quite slowly and has its own limitation. Recently since the decoding of rice genome, many functional genomic tools are available, especially for T-DNA insertional mutagenesis rice mutant pools. This project is a sub-project of functional genomics study of rice development and abiotic stresses with T-DNA tagging rices. In past, we and other teams had finished the GUS staining analysis of T-DNA mutants under different abiotic stresses. Also, we set up PCR-based screening of homozygous rice mutants and established the condition for phenotypic screening. Except continuous to analysis the physiological characteristics of abiotic stress related rice mutants found by other teams, the main goal of this project will focus on physiological and functional characterization of several .T-DNA lines, including 8817 (heat shock protein 83), 27407 (CPEP phosphonomutase), 28360 (LM heat shock protein), 29006 (UDP-glucose 4-epimerase), 34229 (WRKY43)及48000(cytochrome P450). The gene expression pattern, the physiological and biochemical responses and the agronomical traits of these mutants under various abiotic stresses will be determined. Results obtained from these researches are expected to increase our current understanding of rice abiotic stress tolerance mechanism, and can be used further for development of newly abiotic stress tolerant rice cultivars.水稻突變體非生物性逆境T-DNA插入功能性分析rice mutantsabiotic stressT-DNA insertionfunctional analysis