2017-07-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/681890摘要：目前，再生醫學研究大部分仍處於實驗階段。再生醫學與細胞治療的有效性和安全性評估需要在適用於人類患者之前進行徹底的調查。與最常使用的嚙齒類動物疾病模式相比，若考慮到器官大小，解剖構造和生理參數等因素，豬隻動物模式與人類患者的相關與相似性更為密切，因此豬隻是進行細胞治療評估的合適動物模型。目前尚未有人建立用於腹膜損傷和自體間皮細胞治療評估的豬隻動物模式，因此，通過建立豬的動物模式，我們將能夠加速尿毒症患者接受腹膜透析或（自體）間皮細胞治療的測試，並評估治療過程的效果。此計畫旨在建立豬隻動物模式，評估（自體）間皮細胞治療的有效性，安全性和治療過程可能導致腫瘤發生的可能性。此計畫將結合醫學院與獸醫專業學院經驗豐富的團隊（包括3名獲得許可的獸醫，病理學家和外科醫生）在NTU校總區的獸醫學校和動物教學醫院進行）。豬隻動物模式的建立，將如先前在囓齒動物物種中所述，以0.05％次氯酸鈉誘導腹膜損傷，腹膜損傷模型的成功與否，主要將通過組織學檢查來評估，包括內皮襯裡的完整性， 內膜緊密帶厚度，血管病變，炎性細胞浸潤和腫瘤形成。通過建立豬腹膜損傷和評估模型，我們將來可以進行腹膜透析，並評估在good mamagement practice (GMP)級人血小板裂解物中生長的豬間皮細胞是否可以自動轉移（移植回到同一個體的豬）以維持腹膜功能。<br> Abstract: Currently, regenerative medical research is mostly still in the experimental stage. Effectiveness and safety evaluation require thorough investigation prior to apply on human patients. Compared with commonly used murine models, considering organ size, anatomical and physiological aspects, porcine model is more closely related to human patients, and is therefore a suitable animal model for conducting cell therapy evaluations. No current porcine model has been established for peritoneal injury and autologous mesothelial cell therapy evaluations, by establishing porcine model, we will be able to accelerate testing, evaluation processes and clinical applications for (autologous) mesothelial cell therapy in uremic patients undergo peritoneal dialysis. This subproject aim to establish porcine model to evaluate the effectiveness, safety and tumorigenesis potency of mesothelial cell therapy, and will be conducted at the veterinary school and animal teaching hospital at NTU main campus by an experienced team (including 3 licensed veterinarians, pathologist and surgeon). Peritoneal injury will be induced with 0.05% sodium hypochlorite as the method described previously in rodent species. The success of peritoneal injury model will be evaluated by histological examination including the lining of mesothelium, the thickness of submesothelial compact zone, vasculopathy, inflammatory cell infiltration, and tumor formation. By establishing porcine peritoneal injury and evaluation model, we will in the future be able to conduct peritoneal dialysis and evaluate whether porcine mesothelial cells grown in good management practice grade human platelet lysate can be transferred autologusly (porcine to porcine, same individual) to maintain peritoneal function.豬腹膜透析間皮細胞治療動物評估模式porcineperitoneal dialysismesothelial cell therapyanimal model