Chia, M.-Y.M.-Y.ChiaHsiao, S.-H.S.-H.HsiaoChan, H.-T.H.-T.ChanYI-YIN DOHuang, P.-L.P.-L.HuangHUI-WEN CHANGTsai, Y.-C.Y.-C.TsaiLin, C.-M.C.-M.LinVICTOR FEI PANGCHIAN-REN JENG2018-09-102018-09-102011http://www.scopus.com/inward/record.url?eid=2-s2.0-79952183287&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/362771Escherichia coli heat-labile enterotoxin B subunit (LTB) can be used as an adjuvant for co-administered antigens. Our previous study showed that the expression of neutralizing epitope GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) in transgenic tobacco plant (GP5-T) could induce PRRSV-specific immune responses in pigs. A transgenic tobacco plant co-expressing LTB and PRRSV GP5 as a fusion protein (LTB-GP5-T) was further constructed and its immunogenicity was evaluated. Pigs were given orally three consecutive doses of equal concentration of recombinant GP5 protein expressed in leaves of LTB-GP5-T or GP5-T at a 2-week interval and challenged with PRRSV at 7 weeks post-initial immunization. Pigs receiving LTB-GP5-T or GP5-T developed PRRSV-specific antibody- and cell-mediated immunity and showed significantly lower viremia and tissue viral load and milder lung lesions than wild type tobacco plant (W-T). The LTB-GP5-T-treated group had relatively higher immune responses than the GP5-T-treated group, although the differences were not statistically significant. ? 2011 Elsevier B.V.GP5; LTB; PRRSV; Transgenic tobacco leaves[SDGs]SDG3antibody; epitope; Escherichia coli enterotoxin; glycoprotein; protein subunit; recombinant protein; animal cell; animal experiment; animal tissue; antibody production; antibody specificity; article; cellular immunity; concentration (parameters); Escherichia coli; immune response; immunogenicity; lung lesion; male; nonhuman; nucleotide sequence; plant leaf; procine reproductive and respiratory syndrome virus; protein expression; respiratory virus; swine; tobacco; viremia; virus load; Adjuvants, Immunologic; Administration, Oral; Animals; Antibodies, Viral; Bacterial Toxins; Base Sequence; DNA Primers; Enterotoxins; Escherichia coli; Escherichia coli Proteins; Lymphocyte Activation; Male; Plant Leaves; Plants, Genetically Modified; Porcine Reproductive and Respiratory Syndrome; Porcine respiratory and reproductive syndrome virus; Recombinant Fusion Proteins; Saliva; Sus scrofa; Swine; Tobacco; Vaccines, Edible; Vaccines, Synthetic; Viral Envelope Proteins; Viral Load; Viral Vaccines; Escherichia coli; Nicotiana obtusifolia; Nicotiana tabacum; Porcine respiratory and reproductive syndrome virus; Suidaejournal article10.1016/j.vetimm.2011.01.002