2008-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/683202摘要:非生物逆境造成世界上農業方面廣泛的損失。這些個別的生物逆境,例如:乾旱,鹽或者高熱,都受到熱烈的研究。雖然已經累積了大量的知識,但是仍然有許許多多對逆境反應的基因,仍然沒有被研究過。在本團隊計劃中,我們將對乾旱與熱逆境相關的基因或蛋白質進行基礎的研究。在子計劃當中,有五個將探討乾旱逆境(子計劃:1, 2, 3, 4,和5),兩個與熱逆境有關(子計劃6和7),一個與病毒逆境有關(子計劃5)。我們將使用功能性基因體,蛋白質體,結構生物資訊與植物生理的方法來研究這些特殊基因的功能。七個子計劃所用的研究方法實際上是互補的。乙烯反應因子 (ERF)家系已經被研究過知道它們參與了植物對生物與非生物逆境的反應。因此,這個家系是非常珍貴的資源,因為它有潛能可用於改善植物對逆境的耐受力。我們有四個子計劃,希望研究ERF家系的基因,即AtERF4基因的功能(子計劃2)與AtERF4蛋白質的結構(子計劃4),At2g20880在耐旱方面的功能(子計劃1)和At5g61600在乾旱與病毒防禦的功能(子計劃5),這些都會加強子計劃之間的凝聚力與合作關係。我們從未形成如此一個堅強的團隊,針對一個共同的興趣,這將強化我們在植物逆境基礎研究方面的成果,並且對作物改良有所貢獻。<br> Abstract: Abiotic stress conditions cause extensive losses to agricultural production worldwide. Individually, stress conditions such as drought, salinity or heat have been the subject of intense research. Though a great deal of knowledge has been advanced, a large array of genes responsive to stresses is still unknown and unstudied. In this team project we would like to study the basic aspect of dehydration and heat stresses related genes and proteins. Five projects deal with the dehydration stress (sub-projects 1, 2, 3, 4 and 5), two with heat stress (sub-projects 6 and 7), and one with pathogen stress (sub-project 5). We will use functional genomics, proteomics, structure bioinformatics and physiological approach to study the specific gene function. The approaches used by the 7 sub-projects are complementary. The ethylene response factors (ERFs) family members that have been functionally characterized are involved in plant responses to biotic or abiotic stresses. Hence, this family seems to be a valuable resource to evaluate in terms of its potential for engineering plant stress tolerance. We have four sub-project want to study ERF family genes, i.e., The function of AtERF4 gene (sub-projects 2) and protein structure of AtERF4 (sub-project 4), the function of At2g20880 in dehydration (sub-projects 1) and the function of At5g61600 in dehydration and pathogen defense (sub-projects 5). This will enhance coherence and cooperation among sub-projects for the common interest. We have never formed such a team strong enough like this one working on a defined biological problem which will strengthen our basic research while has a potential to contribute to agriculture improvement.阿拉伯芥番茄ERF家系缺水熱逆境蛋白質體學病毒防禦Arabidopsis thalianaERF familywater deficitheat stressproteomicspathogen defense利用模式植物進行逆境生物學之功能性基因體與蛋白質體研究