2014-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/678416摘要：類尼古丁殺蟲劑被廣泛地應用於農業蟲害防治，然而此類藥劑極可能造成蜜蜂導航神經系統及外出採集行為的異常，進一步導致田間的蜂群衰竭失調症。為驗證此一假說，本研究以亞致死劑量的益達胺餵飼蜂群，人為方式誘發蜂群衰竭失調症；經由直接監測受試蜂群工蜂外出採集行為的變化，及應用電生理記錄技術、免疫標定及行為試驗等方法研究益達胺毒害干擾蜜蜂導航系統的狀況，探討蜂群衰竭失調症的發生機制。同時本計劃亦將進行蜜蜂磁感受神經電生理及其相關神經網絡的研究，增進對蜜蜂磁感受機制的認知，以延伸益達胺危害評估涵蓋的層面。預期藉由本研究的相關研究成果，將可釐清類尼古丁殺蟲劑對蜂群的影響，及在田間發生蜂群衰竭失調症時所扮演的角色。<br> Abstract: Neonicotinoids, a group of widely used agricultural pesticides, have been suspected inducing the colony collapse disorder (CCD) of honeybees by disordering the navigation system and/or disrupting their foraging behavior. In this study, we will examine this hypothesis by evaluating the toxicity of imidacloprid, a neonicotinoid insecticide. Honeybee colonies will be treated with sub-lethal dosage of imidacloprid for a period of time to induce the symptom of CCD artificially. The imidacloprid toxicity effect on the forging behavior will be investigated by monitoring the short-term and long-term effects of foraging properties, such as the duration and frequency of foraging behavior. By electrophysiological recording, imidacloprid-immunostaining and behavioral assay, the imidacloprid toxicity effect on the navigation system will be studied. Furthermore, by exploring the neural processing and the architecture of the magnetoreception system of honeybee, this study will reveal the mechanism of magnetoreception, one of the main navigational cues, in the honeybee. The study of magnetoreception will provide evidences to clarify whether the CCD is caused by navigation disorder and neonicotinoid toxicity. The key factors of the imidacloprid-induced CCD will be discovered in this study, and it can therefore be concluded that whether the neonicotinoids cause CCD in the field and how this chemical would contribute to the syndrome.蜂群衰竭失調症導航磁場感應類尼古丁殺蟲劑蜜蜂colony collapse disorderCCDnavigationmagnetoreceptionneonicotinoidshoneybeeApis mellifera前瞻研究領航計畫【新型殺蟲劑對蜜蜂導航神經系統之影響】