2018-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/702903摘要：台灣畜牧業為了增加收益，常在有限的面積內密集飼養家畜禽，導致疾病的發生率較高。畜牧業常見的疾病中，由病毒引起的疾病極為普遍。2011年台灣的豬隻疾病檢診病例中，豬生殖與呼吸症候群病毒（porcine reproductive and respiratory syndrome virus；PRRSV）發生率最高，佔所有豬隻疾病的13%；其次為豬環狀病毒第二型（Porcine circovirus type 2；PCV2），佔11%；而兩者混合感染所佔的比例更高達49%。禽鳥的病毒性疾病中，則以禽流感最為令人聞之色變，2015年台灣禽鳥之禽流感發病數已近100萬隻，更因此而撲殺163萬隻禽鳥，故禽流感已成為台灣家禽業首要的威脅。為減少病毒性疾病對畜牧業造成的危害與經濟損失，本計畫擬針對乳酸菌抗病毒製劑進行開發與研究。計畫中將分離豬隻與雞隻之腸道乳酸菌，並以具有抗病毒活性之菌株為主要篩選目標，期能篩選出具有抗病毒活性之腸道益生菌。另將進一步以腸道益生菌做為黏膜載具，使其表達干擾素α與禽流感病毒H5N1之HA蛋白柄狀區域，預期此些基因重組乳酸菌能於動物消化道誘發抗病毒相關基因的表達，或產生抗禽流感病毒的專一性抗體。本計畫篩選所得之具抗病毒活性的腸道益生菌及其基因重組菌株將可開發為畜牧用抗病毒製劑。 <br> Abstract: In order to increase the swine and poultry production, it’s commonly to raise animals in areas of high-density population. Therefore, the infectious disease could spread easily and rapidly. Among the infectious disease, viral diseases play a great role in yield reduction. In the swine industry in Taiwan, porcine reproductive and respiratory syndrome virus (PRRSV) is the most widespread disease, followed by Porcine circovirus type 2 (PCV2). In the poultry industry, outbreaks of avian influenza cause devastating economic losses. More than one million birds were infected by avian influenza virus and about 1.6 million birds were forced to slaughter in 2015 in Taiwan. Therefore, it’s need to develop a new strategy for the treatment of viral disease. This project is conducted to develop antivirus agents based on lactic acid bacteria. The lactic acid bacteria will be isolated from the intestinal tract of swine and broilers, and the stains possess antiviral activities will be screened out. The strains with probiotic activity will further be used to express the genes encoding interferon-α and hemagglutinin (HA) of influenza A (H5N1). The expression of the interferon-induced antiviral proteins and the production of the specific anti-HA antibodies in the animal intestinal tract will be determined. The probiotic strains developed in this project are expected to have potential to be used as antiviral feed additives.益生菌病毒干擾素α家禽流行性感冒血球凝集素probioticvirusinterferon-αavian influenzahemagglutinin