Liu, Yen WennYen WennLiuLiu, Chi MeiChi MeiLiuChen, Hung YuehHung YuehChenKhumsupan, DarinDarinKhumsupanHsu, Hsien YiHsien YiHsuLin, Hui WenHui WenLinHsieh, Chang WeiChang WeiHsiehKUAN-CHEN CHENG2023-05-092023-05-092023-04-012311-5637https://scholars.lib.ntu.edu.tw/handle/123456789/630935Ganoderma formosanum is a medicinal mushroom endemic to Taiwan. The extraction of G. formosanum mycelium using ethyl acetate showed a significant reduction of melanin activity due to the inhibition of tyrosinase. To optimize the production of G. formosanum mycelium with anti-melanogenic activity, different variables (carbon sources and concentrations, initial pH value, and temperature) were investigated. According to the results, the optimal conditions included the utilization of 50 g/L lactose as the carbon source at pH 7 and 25 °C for 9 days. Afterward, the G. formosanum ethanolic extracts-optimized (GFE-O) was used to study the anti-melanogenic activity in vitro and in vivo. In B16-F10 melanoma cells treated with GFE-O (0.1 mg/mL), the intracellular melanin content was reduced to 76% compared to the control group. By applying GFE-O (0.05 mg/mL) in vivo on zebrafish embryo, hypopigmentation was observed, and the melanin content was reduced to 62% compared to the control with no toxicological effects. The results showed that the optimal inoculation conditions can provide the basis for future large-scale production of G. formosanum mycelium to maximize the extraction of anti-melanogenic ingredients.Ganoderma formosanum | medium optimization | mycelium | skin-lightening activity | tyrosinase inhibition[SDGs]SDG3journal article10.3390/fermentation90403722-s2.0-85153953584https://api.elsevier.com/content/abstract/scopus_id/85153953584