2017-01-092024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/656406摘要：土壤重金屬污染是一個全球性的污染問題，其中六價鉻（Cr(VI)）為一廣泛存在的重金屬污染物，在台灣的一些土壤污染列管場址就含有Cr(VI)，其毒性及遷移性都遠大於三價鉻（Cr(III)）。Cr(VI) 污染的大量存在會對人體健康和生態安全產生潛在的危害，並是一疑似致癌物。植生復育（phytoremediation）是近年開發的一種環境友善的新興原位復育技術，可通過植物的生長和對重金屬的吸收，為重金屬污染的土壤復育開闢了一個途徑。比較理想的植生復育是利用重金屬高聚積植物吸收污染環境中的重金屬，然後收割植株將污染物帶離污染土壤。然而對於鉻而言，至今發現的鉻高聚積植物非常有限，此外這些發現的鉻高聚積植物生物量小，限制了其在鉻植物修復中的應用。 微生物電化學技術（例如我們常聽到的微生物燃料電池）為一新興的環境生物技術，由微生物和電極系統組成，利用產電微生物降解有機污染物同時，進行胞外電子傳遞到電極上，藉此可以達到污染治理及能源收集的目的。微生物電化學技術在經過了最近十多年的發展，已經開發出不同的應用系統，可以達到產氫、產甲烷、產雙氧水、脫鹽及還原重金屬等應用。最近也開發出耦合植物及微生物電化學技術的植物微生物燃料電池系統，利用植物根系分泌物，提供根系的微生物包含產電菌作為碳源，促進微生物胞外電子傳遞，結合電極系統，可以收集能源。 本研究計畫之目的在於評估以植物微生物燃料電池技術復育Cr(VI)污染土壤，提升對Cr(VI)的去除效果，同時並能產能。研究內容包含二階段，首先構建基於蘆葦的植物微生物燃料電池系統，並利用蘆葦對Cr(VI)的吸收及微生物電化學系統對Cr(VI) 在電極上的還原及沉積的協同作用，達到促進Cr(VI)在土壤中的去除效果及產能。第二階段我們將在第一階段的基礎上更進一步進行實際污染土壤的測試，期待能加速Cr(VI)在污染土壤中的去除效果。本研究的成果具有學術貢獻與應用價值，可突破傳統Cr(VI)污染土壤處理技術之瓶頸，並降低對於民眾健康的潛在危害風險。 <br> Abstract: Heavy metals contaminated soil is a global pollution problem. Cr(VI) is a widely reported heavy metal contaminant and it has much higher toxicity and mobility than Cr(III). Some reguratory sites in Taiwan contain Cr(VI). Widespread Cr(VI) contamination will cause serious threat to the health of human and ecosystem. It is also a potential carcinogen. Phytoremediation is an environmentally-friendly in-situ remediation technique in recent years and can remove heavy metals through the uptake by plants. Therefore, it provides a new choice for the remediation of heavy metals contaminated soil. It is ideal to use the hyperaccumulator plant to uptake heavy metals during the plant growth and harvest plants to remove heavy metals from soil. However, there are very few reported hyperaccumulator plants for Cr, and these plants are relatively small, which limits the application of phytoremediation of Cr. The microbial electrochemical system (MES, e.g. microbial fuel cells (MFC)) is an emerging environmental biotechnology. It is composed of microorganisms and electrodes system. When microorganisms metabolize organic pollutants, extracellular electron transfer (EET) to the electrode will occur, and therefore, can simultaneously achieve pollutant treatment and electricity collection. After the development during the past decade, scientists have invented different MES system (MxCs), for example, to produce H2, CH4, and H2O2, to desalinate water, to reduce heavy metals. Recently, researchers have integrated plants into the MES system, which is named plant microbial fuel cell (PMFC). In PMFC, plant’s root will produce exudate, and bacteria including exoelectrogens can use the exudate from roots as the carbon source and achieve EET and transfer electrons to the electrode for electricity production. In this proposal, we intend to evaluate the use of PMFC for bioremediation of Cr(VI) contaminated soil and also produce energy at the same time. Study will be devided into two parts: First, we will build a PMFC based on Phragmites australis. We will use Phragmites australis’s ability to uptake Cr(VI) and also use MES system to reduce Cr(VI) to Cr(III) and precipitate Cr(III) on the electrode. We hope the integrated mechanisms can enhance Cr(VI) removal in comtaminated soil and produce energy. Second, on the basis of the first part of study, we planned to test by using soil from the contaminated site. In this way, we hope to further improve Cr(VI) removal in contaminated soil. The research in this proposal can improve the current knowledge of treatment techniques in Cr(VI) removal, providing useful information for the potential filed applications of Cr(VI) contaminated sites.植生復育生物電化學系統植物微生物燃料電池鉻PhytoremediationBioelectrochemical systemPlant Microbial Fuel CellChromium