Lee, Tsung HanTsung HanLeeTsai, Jong ChangJong ChangTsaiFang, Mei JaneMei JaneFangMING-JIUN YUHwang, Pung PungPung PungHwang2020-01-232020-01-231998-10-0703636119https://scholars.lib.ntu.edu.tw/handle/123456789/452957Three isoform-specific antibodies, 6F against the α1-isoform of the arian sodium pump, HERED against the rat α2-isoform, and Ax2 against the rat α3-isoform, were used to detect the expression of Na+-K+-ATPase α- subunits in gills of a teleost, the tilapia (Oreochromis mossambicus). Tilapia gill tissue showed positive reactions to antibodies specific for α1- and α3-isoforms. The results of immunoblots were converted to numerical values (relative intensities) by image analysis for comparisons. Relative amounts of α1-like isoform alone and consequently the ratio of α1-like to α3-like isoforms were higher in gills of seawater-adapted tilapia than in those of freshwater-adapted ones, indicating that the two isoforms respond differently to environmental salinities. In the subsequent immunocytochemical experiments, gill mitochondria-rich cells were demonstrated to immunoreact with antibodies specific for α1- and α3- isoforms. α1-like and α3-like isoforms of gill Na+-K+-ATPase are suggested to be involved in the ion- and osmoregulation mechanisms in tilapia. Moreover, differential expressions of two isoforms may be associated with different functions, secretion and uptake of ions and acid-base regulation, in gills of seawater- and freshwater-adapted tilapia.enChloride cell | Ion regulation | Sodium pump[SDGs]SDG14journal article10.1152/ajpregu.1998.275.3.R92697280932-s2.0-0031704888https://api.elsevier.com/content/abstract/scopus_id/0031704888