TSUNG-LIN YANGLin LPEI-JEN LOUChang T.-CTai-Horng Young2020-10-272020-10-2720141932-6203https://www.scopus.com/inward/record.uri?eid=2-s2.0-84900305192&doi=10.1371%2fjournal.pone.0086143&partnerID=40&md5=dfb02e2288454bf16d036b0295ff6c48https://scholars.lib.ntu.edu.tw/handle/123456789/518229Cancer can be easily treated when found early. A probe capable of detecting cell transformation may increase the success rate of early diagnosis of cancer. In this report we have tested the ability of a fluorescent, quadruplex DNA binding probe, 3,6-bis(1-methyl-4- vinylpyridinium) carbazole diiodide (BMVC), to detect cell transformation in vitro. BMVC was applied to living cells in several different models of cell transformation, and the fluorescence signals of BMVC were measured. The degrees of cell transformation in these models were characterized by alterations in cellular morphological phenotype and subcellular organization. When BMVC probes were applied, the number of BMVC-positive cells increased in accordance with the degree of transformation. BMVC was capable of significantly detecting formation of foci, increased cellular motility, cell proliferation, cell apoptosis, anchorage-independent growth, and increased invasiveness of transformed cells. These results demonstrate the ability of BMVC probes to detect cell transformation and indicate that BMVC is of promise for use as a probe in early cancer detection. ? 2014 Yang et al.[SDGs]SDG33,6 bis(1 methyl 4 vinylpyridinium)carbazole diiodide; fluorescent dye; unclassified drug; fluorescent dye; guanine quadruplex; anchorage independent growth; animal cell; apoptosis; article; cancer cell; carcinogenesis; cell invasion; cell migration; cell motility; cell proliferation; cell structure; cell transformation; colony formation; controlled study; fluorescence analysis; in vitro study; mouse; nonhuman; animal; cell line; cell transformation; chemistry; flow cytometry; fluorescent antibody technique; human; metabolism; spectrofluorometry; Animals; Cell Line; Cell Transformation, Neoplastic; Flow Cytometry; Fluorescent Antibody Technique; Fluorescent Dyes; G-Quadruplexes; Humans; Mice; Spectrometry, Fluorescencejournal article10.1371/journal.pone.0086143244896942-s2.0-84900305192