SUNG-HSIN KUOChen L.-T.Chen C.-L.SHIN-LIAN DOONGKUN-HUEI YEHMING-SHIANG WUTSUI-LIEN MAOHsu H.-C.HSIU-PO WANGLin J.-T.ANN-LII CHENG2020-03-072020-03-0720051007-9327https://www.scopus.com/inward/record.uri?eid=2-s2.0-23244455747&doi=10.3748%2fwjg.v11.i28.4357&partnerID=40&md5=7d8cfa699c300544f1e2e7f3120cdc07https://scholars.lib.ntu.edu.tw/handle/123456789/473658Aim: A high percentage of early-stage high-grade gastric mucosa-associated lymphoid tissue (MALT) lymphomas remain Helicobacter pylori (H pylori)-dependent. However, unlike their low-grade counterparts, high-grade gastric MALT lymphomas may progress rapidly if unresponsive to H pylori eradication. It is mandatory to identify markers that may predict the H pylori-dependent status of these tumors. Proliferation of MALT lymphoma cells depends on cognate help and cell-to-cell contact of H pylori-specific intratumoral T-cells. To examine whether the expression of co-stimulatory marker CD86 (B7.2) and the infiltration of CD56 (+) natural killer (NK) cells can be useful markers to predict H pylori-dependent status of high-grade gastric MALT lymphoma. Methods: Lymphoma biopsies from 26 patients who had participated in a prospective study of H pylori-eradication for stage IE high-grade gastric MALT lymphomas were evaluated. Tumors that resolved to Wotherspoon grade II or less after H pylori eradication were classified as H pylori-dependent; others were classified as H pylori-independent. The infiltration of NK cells and the expression of CD86 in pre-treatment paraffin-embedded lymphoma tissues were determined by immunohistochemistry. Results: There were 16 H pylori-dependent and 10 H pylori-independent cases. CD86 expression was detected in 11 (68.8%) of 16 H pylori-dependent cases but in none of 10 H pylori-independent cases (P = 0.001). H pylori-dependent high-grade gastric MALT lymphomas contained significantly higher numbers of CD56 (+) NK cells than H pylori-independent cases (2.8±1.4% vs 1.1±0.8%; P = 0.003). CD86 positive MALT lymphomas also showed significantly increased infiltration of CD56 (+) NK cells compared to CD86-negative cases (2.9±1.1% vs 1.4±1.3%; P = 0.005). Conclusion: These results suggest that the expression of co-stimulatory marker CD86 and the increased infiltration of NK cells are associated with H pylori-dependent state of early-stage high-grade gastric MALT lymphomas. ? 2005 The WJG Press and Elsevier Inc. All rights reserved.[SDGs]SDG3amoxicillin; antineoplastic agent; bismuth citrate; CD56 antigen; CD86 antigen; clarithromycin; metronidazole; omeprazole; adult; aged; antigen expression; article; cancer grading; cancer growth; cancer staging; cell contact; cell proliferation; clinical article; controlled study; female; Helicobacter infection; Helicobacter pylori; human; human cell; human tissue; immunohistochemistry; lymphocytic infiltration; male; mucosa associated lymphoid tissue lymphoma; natural killer cell; prediction; prospective study; stomach biopsy; stomach lymphomajournal article10.3748/wjg.v11.i28.4357160380342-s2.0-23244455747