2018-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/677650摘要：計畫宗旨將基礎研究中植物耐淹水機制轉譯至甘藍。以抗耐性的分子機制為基礎，若將不同機制組合在單一植物個體上，便育成更淹水耐品系。為了填補台灣夏季栽培的市場缺口，育成更耐受的甘藍品種為必要方法。然而，缺少甘藍淹水耐性的知識基礎時，育種效率相對低落。本計畫將解析耐性機制之基礎，再配合本團隊建構中的甘藍分子標誌輔助選種平台，預期甘藍的育種可有效的加速，用以面對氣候變遷與市場需求。在此，本團隊設計了兩階段式的策略。第一階段將已知的耐淹水基礎知識轉譯至甘藍，第二階段整合代謝體與轉錄體資訊，用以闡釋耐淹水機制。此外，我們的初步研究已找到耐與不耐淹水的甘藍品種。然而，目前甘藍研究還未有採取類似策略。為了達成上述策略，我們假設多數模式植物得出的耐淹水機制可以轉譯至甘藍中。為測試假設，特定目標為：一、剖析缺氧核心基因與調節機制相關基因之表現; 二、鑑定氧化逆境與能量缺乏程度; 三、以代謝體工具剖析缺氧相關之代謝物; 四、以轉錄體工具鑑定差異化表現基因。此目標有新創性，因該案為轉譯耐淹水基礎研究到蔬菜的首例。此研究計畫也有顯著性，因其垂直應用基礎知識推進台灣農業研究，且最終能幫助臺灣更新育種技術與改善甘藍生產。<br> Abstract: The major goal of this project is to translate knowledge of plant flooding tolerance mechanisms form basic research into cabbage. Based on dissected molecular mechanisms of tolerance, combination of tolerant mechanisms into an individual plant is able to breed new varieties with higher flooding tolerance. To fill the cabbage production gap in warm season in Taiwan, new cabbage breeds that have stronger flooding tolerance must be selected. However, lack of knowledge basis for flooding tolerance in cabbage would decrease the efficiency of breeding. Using such knowledge basis that will obtain in this proposal and taking advantages of the marker-assisted selection (MAS) platform for cabbages that is developing in our research team, cabbage breeding processes are expected to significantly accelerate in order to confront the climate change and the market demand. Here, we designed a strategy consisted of two stages. The first stage is to translate knowledge of flooding tolerance mechanisms from well-known basic research in model plants into cabbage, and the second stage is to integrate metabolomic and transcriptomic information for interpreting flooding tolerance mechanisms. Moreover, our preliminary data have found flooding-tolerant and flooding-intolerant cultivars for further analysis. However, such strategies have not been studied in cabbage. To approach this strategies, we hypothesized that most mechanisms of flooding tolerance studied in model plants is able to translate into cabbage. To test this hypothesis, our specific aims are: (i) to profile transcript levels of hypoxic core genes and genes associated with regulatory mechanisms, (ii) to determine oxidative stress and energy starvation level upon submergence and after reoxygenation, (iii) to profile levels of hypoxia-associated metabolites using metabolomic approach, and (iv) to identify differentially expressed genes (DEGs) using transcriptomic approach. The aims in our proposed study is innovative, because it would be the first case to translate knowledge of flooding tolerance form basic research into vegetables. The proposed research is significant, because it is expected to vertically apply knowledge into agriculture applications on advancing technology of agricultural research for Taiwan, and ultimately will help to update breeding technology and reduce production problems of cabbage in Taiwan.轉譯農學甘藍淹水耐受性轉錄體學代謝體學translational agriculturecabbageflooding tolerancetranscriptomicsmetabolomics