張璞曾臺灣大學:電機工程學研究所王正邦Wang, Jeng-PangJeng-PangWang2010-07-012018-07-062010-07-012018-07-062009U0001-2007200919452800http://ntur.lib.ntu.edu.tw//handle/246246/188000此論文提出一個人類健康長壽及其風險的免疫發炎模型。發炎導致大多數疾病（癌症，動脈粥樣硬化，老化相關疾病），為疾病共同引發途徑和主要風險，並導致最多病痛和死亡。論文模型，強調用非化學的抗發炎措施預防和治療疾病的可行性：消除食物抗原，食物毒素；解除精神壓力；消除隱性感染；和維持一個有效率的先天免疫。我們通過非化學非侵入性的方法，使用電子生物反饋，作為一種對抗發炎的措施。電子生物反饋抗炎反射效率很高，兼顧非化學非侵入性，為此一做法在每個人日常生活用以預防疾病，加強了基礎。文提出可能大幅延長人類無病痛之壽命的理論。用目前已有的自體先天免疫NK細胞增殖技術改變免疫表現型（immune phenotype）成為NK細胞優勢表現型，可能可以延後人類疾病，癌症，動脈粥樣硬化，老化發生, 減少病痛和死亡，大幅延長可能壽命。 天免疫細胞治療癌症延長人類壽命的案例研究證明，利用自己的先天免疫治療癌症是可行的，合理的。四個末期癌症案例研究中。一末期肝癌患者，以自體先天免疫細胞NK細胞輸血作為唯一的治療。 肝癌及門靜脈血栓完全消失！自體NK細胞輸血延長所有末期癌症案例的生存，延長無症狀間隔，延長病程無惡化間隔。它可以與任何療法組合使用。病人壽命生活品質大幅優於其他未併用NK治療方式的末期癌症患者。體先天免疫細胞NK細胞的免疫表現型優勢地位，不僅有助於治療癌症是，也有助於預防動脈粥樣硬化。在冠心病豁免人口中，自然殺手細胞（NK）的CD 56暗淡子型（CD 56 Dim subtype）是優勢免疫表現型。相反的，冠心病患者免疫表型中的，自體先天免疫細胞NK細胞表現劣勢。NK細胞是健康長壽的免疫表型。具NK細胞的優勢免疫表現型的老人有較佳內分泌樣貌，較多的肌肉，生活較活躍，較少感染發炎，較少病痛，較長壽。K細胞治療和預防癌症。NK細胞可以防止動脈粥樣硬化。NK細胞協助治療各種感染，使發炎重回到平靜。NK細胞的優勢表型的是健康老齡化。靜脈周血有容易取得到豐富的NK細胞前體細胞，使先天免疫細胞NK細胞的優勢免疫表現型可以輕易達成。化學抗發炎，以及非化學自體先天免疫細胞NK細胞解決方案，將是一個非常重要的防止病痛和死亡的方法。每個人在日常生活中對解除免疫負荷，解除精神壓力，非化學抗炎措施，達成自體先天免疫細胞NK優勢表現型，將有效地降低癌症，動脈粥樣硬化，許多老化有關的疾病的發生。待免疫的生活方式，降低發炎，優先使用對免疫無害的有效方法治療疾病，並促進或改變成為先天免疫細胞NK優勢表現型，人類壽命將有可能大幅超越目前的限制。In this thesis we propose an immune –inflammatory model of human longevity and risks. This thesis will theorize that inflammation as a common pathway and major risk leading to most diseases ( cancer, atherosclerosis, aging-related diseases).and leading to most morbidity and mortality. Anti-inflammatory measures are going to slow or prevent diseases from major health risk, inflammation. And the thesis will also theorize immune phenotype change by available cell proliferation technology would extend current limitation of human longevity.here are many non-chemical approaches in disease prevention and treatments. Of these, electrical bio-feedback is an efficient non-invasive non-chemical approach against inflammation. The nature of non-invasiveness and the efficiency of this approach strengthens the basis for using electrical bio-feedback anti-inflammatory reflex in disease prevention for everyone in daily life. Here, the result showed that HRV-HR is a promising parameter for checking inflammation. Other non-chemical approach are also emphasized in disease prevention and treatment: Eliminate of food antigen, food toxins; Unload mental stress; Eradicate occult infection. And maintain an efficient innate immunity.his thesis also showed that changing immune phenotype is available by cell proliferation technology. The case study on cancer innate immune therapy proves that it is possible and reasonable to use our own innate immunity to treat cancer. Autologous natural killer( NK ) cell transfusions eradicate cancer in pilot study. Terminal hepatocellular carcinoma with portal vein thrombosis totally disappeared with NK cell transfusion as sole treatment. NK cell transfusion prolongs survival in all cases. NK cell transfusion lengthens symptom-free intervals and progression free intervals. It is freely used in any combination therapy. The life quality of patient is much superior to otherwise treated patients. The phenotype change toward NK cell dominance is not only useful in treating cancer is is also useful in prevention against atherosclerosis.The phenotype of NK cell, CD 56 dim played an important role in Coronary Heart Disease. This type of patients has less NK cells. NK is the immune phenotype of healthy longevity. Those with higher NK has favorable endocrine profile, more muscle, more life activity, and less morbidity. Moreover, NK treats and prevents cancer, atherosclerosis, treats variety of infection and brings inflammation back to homeostasis. Abundant NK precursor cells are easily available in the peripheral blood. NK treats and prevents cancer. NK prevents atherosclerosis. NK cell treats variety of infection and brings inflammation back to homeostasis. NK prevalent is the phenotype of healthy ageing. There is easily available abundant NK precursor cells in the peripheral blood , that make change into NK phenotype type present and practical by current proliferation or promotion technology. Thus, we conclude that by having your life immune-friendly way, by lowering the inflammation to prevent diseases, by selecting effective and immune-harmless treatment against diseases, and by promoting or changing into NK prevalent immune phenotype, would be the key for the human being to live beyond the current limitation of human longevity.TABLE OF CONTENTS PageCKNOWLEDGMENTS..............................................................................i要...............................................................................................................iiBSTRACT.................................................................................................ivABLE OF CONTENTS.............................................................................viIST OF FIGURES......................................................................................ixHAPTER 1 INTRODUCTION...................................................................1.1 Background….........................................................................................1.2 Motivation, Objective and Three Questions to be Answered.................2.3 Our Previous Work..................................................................................3.4 Some Progress of Immune Based Cancer Therapy: Cell therapy...........3.5 Dissertation Organization........................................................................4HAPTER 2 AN IMMUNE-INFLAMMATORY MODEL OF HUMAN LONGEVITY AND RISKS..........................................................................6.1 A Few Word Ahead of the Model...........................................................6.2 The Immune- Inflammatory Model.........................................................6.3 Immune Loading.....................................................................................7.3.1 Antigen or Toxin..................................................................................7.3.2 Infection................................................................................................8.3.3 Mental Stress........................................................................................8.4 The Inflammatory Response and the Amplifier......................................8.5 The results of the Inflammation..............................................................9.5.1 Symptom/Sign......................................................................................9.5.2 Biosignal / Clinical Markers.................................................................9.5.3 Diseases from Inflammation..............................................................10.5.4 Ageing from Inflammation.................................................................11.6 How May Use this Model to Prevent Diseases and ExtendLongevity..12HAPTER 3 INFLAMMATION CASE STUDY......................................14.1 Inflammation Associated with Reduced Heart Rate variability............14.2 Anti-Inflammatory Reflex by Electrical Biofeedback(Vagal Stimulation).................................................................................................15.3 Experimental Design.…........................................................................15.4 Signal Processing..................................................................................15.5 Results and Conclusion.........................................................................17HAPTER 4 NK CELL AS THEORY IDEA OF LONGEVITY PHENOTYPE OF LONGEVITY AGAINST CANCER, AGEING, AND ATHEROSCLEROSIS................................................................................19.1 NK Cell of Innate Immunity.................................................................19.2 NK Cell against Cancer.........................................................................21.3 NK Cell against Ageing........................................................................22.4 NK Cell in Longevity and Exempt from Atherosclerosis.....................22.4.1 Longevity and Exempt from Atherosclerosis.....................................23.4.2 NK cell of Innate Immunity and Exempt from Atherosclerosis........23.4.3 NK Cell as Longevity Phenotype.......................................................24.4.4 Why is NK?........................................................................................24HAPTER 5 NK CASE STUDY...............................................................25.1 Lymphocyte Immunophenotyping by Flow Cytometry........................25.2 Fuctional Study and Cloning of NK Cell..............................................27.3 NK Case Study 1 Hepatic Cancer Stage 4............................................28.4 NK Case Study 2 Lung Cancer Stage 4................................................31.5 NK Case Study 3 Pancreatic Cancer Stage 4........................................33.6 NK Case Study 4 Adrenal Gland Cancer Stage 4.................................34HAPTER 6 CONCLUSION.....................................................................35EFERENCE..............................................................................................38UBLICATION LIST.................................................................................411818959 bytesapplication/pdfen-US長壽疾病的發病機制統一的理論免疫發炎抗發炎反射精神壓力心率變異性非化學免疫療法細胞療法自然殺手細胞腫瘤動脈粥樣硬化老化NK細胞優勢免疫表現型longevitydiseases pathogenesisunified theoryimmuneinflammationanti-inflammatory reflexmental stressheart rate variabilitynon-chemicalimmune therapycell therapynatural killer cellcanceratherosclerosisageingNK prevalentimmune phenotype[SDGs]SDG3thesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/188000/1/ntu-98-D89921038-1.pdf