PO-LING KUOHao-Dinh Phung2019-10-312019-10-3120171557170xhttps://scholars.lib.ntu.edu.tw/handle/123456789/429288https://www.scopus.com/inward/record.uri?eid=2-s2.0-85032208423&doi=10.1109%2fEMBC.2017.8037262&partnerID=40&md5=e3668d2fd3bceeb16c1c1db5ff46fd0cHepatic stellate cells (HSCs) play a crucial role in the development of liver fibrosis which is characterized by massive tissue scarring, elevated hepatic sinusoidal pressure, and portal hypertension. However, while a multitude of chemical factors have been investigated in the pathogenesis of liver fibrosis, physical factors such as elevated hydrostatic pressure and shear stress caused by blood flow in sinusoids remain unclear. In this study, we developed a three-dimensional (3D) cell culture microfluidic platform that mimics the physical environments of hepatic sinusoids to investigate the effects of elevated hydrostatic pressure on HSCs phenotypes. © 2017 IEEE.cell culture technique; hepatic stellate cell; human; hypertension; liver; liver cirrhosis; portal hypertension; Cell Culture Techniques; Hepatic Stellate Cells; Humans; Hypertension; Hypertension, Portal; Liver; Liver Cirrhosisconference paper10.1109/embc.2017.8037262290603052-s2.0-85032208423