2009-06-182024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/679625摘要：植物的生長發育主要取決於在莖頂和根尖的分生組織幹細胞活力。莖頂分生組織尤其重要因其掌管著植物器官如葉子和花的發育與分化。正常開花植物的幹細胞只存在莖頂分生組織中，分化後的葉子及花裡分生組織活動應該關閉。然而，我們團隊專注研究的苦苣苔科模式開花植物-堇蘭(Streptocarpus rexii) ，持續活躍的分生組織異位產生於子葉，使得兩片子葉的大子葉可以持續長大，而小子葉則正常的生長到一定程度為止。另外，在苦苣苔科另一模式植物大岩桐的園藝馴化種中，花由野生型的兩側對稱型突變成輻射對稱。這些例子顯示苦苣苔科植物在花及葉中有相當大的發育可塑性。本計畫透過與英國、義大利、日本及加拿大合作團隊的研究分工，利用分析大、小子葉發育間表現基因體的不同，及兩側對稱與輻射對稱型花之間花對稱性基因表現的差異，計畫分離出與子葉持續生長及花對稱性發育的基因，並積極建立轉殖系統等基因功能性研究方法，希望促成未來更深入之各國雙邊合作計畫申請與研究成果發表。<br> Abstract: Shoot and root apical meristem are major parts responsible for plant development as they contain stem cell activities. Shoot apical meristem is particularly important as it determine the differentiation of leaves and flowers. Typically meristem activities should be switched off in leaf and flower primordia. However, in Gesneriaceae model plant species Streptocarpus rexii, meristem activities are ectopically active in cotyledon thus resulting in continuously growing macrocotyledon. This enables us to compare transcription differences between macrocotyledon and microcotyledon to isolate candidate genes in charge of macrocotyledon development. Flower reversions from zygomorphy to actinomorphy has been observed in another model species Sinningia speciosa. By comparing the expression of floral symmetry genes between wild type and peloria shall enable us to find out the developmental switch from actinomorphy to zygomorphy. This pilot project therefore is aim to build up research networks among research teams from Taiwan, Britain, Italy, Japan and Canada to isolate and study the candidate genes function in relation to cotyledon and floral symmetry development. We also collaborate in building up gene transformation systems in these Gesneriaceae species. By sharing expertise and techniques from our worldwide research teams, we hope to promote applying further bilateral research projects and to publish our findings when further funds are available幹細胞分生組織子葉不等大花對稱性AnisocotylyKNOXGibberellinsfloral symmetry